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| 应用FISH技术对肝癌细胞系HCC-9903X染色体变异的研究 | |
| 引用本文： | 刘军,王占民,郑淑芳,李东华,吴小鹏,马道新,李昭晖,刘博,赵志伦,柯扬.应用FISH技术对肝癌细胞系HCC-9903X染色体变异的研究[J].中华普通外科杂志,2003,18(2):105-106. |
| 作者姓名： | 刘军 王占民 郑淑芳 李东华 吴小鹏 马道新 李昭晖 刘博 赵志伦 柯扬 |
| 作者单位： | 1. 210029 南京医科大学第一附属医院肝脏移植中心 2. 250012,济南,山东大学齐鲁医院普外科 3. 北京大学临床肿瘤学院、北京市肿瘤防治研究所 |
| 基金项目： | 山东省自然科学基金资助项目 ( 0 0 3130 10 7) |
| 摘 要： | 目的：研究人肝细胞肝癌细胞系HCC－9903X染色体畸变规律。方法：建立肝细胞肝癌细胞系HCC－9903，制备其染色体标本，用X染色体着丝粒特异DNA探针，对其X染色体进行荧光原位杂交（fluorescence in situ hybridization,FISH)，检测X染色体拷贝致畸变。结果：HCC－9903肝癌细胞系染色体变异发生率高，其中X染色体改变存在规律，拷贝数增多，以4拷贝为主，X染色体扩增率达99％，结论：X染色体拷贝数增多在肝癌细胞系中发生频率很高，证明在肝癌发病及发展过程中起到某种作用，其意义值得进一步探讨。 |
| 关 键 词： | 肝细胞癌 X染色体 荧光原位杂交 |
| 修稿时间： | 2002年7月19日 |
Detection of the aberration of X chromosome in hepatocellular carcinoma cell line HCC-9903 by fluorescence in situ hybridization(FISH) |
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| LIU Jun,WANG Zhan-min,ZHENG Shu-fang,et al..Detection of the aberration of X chromosome in hepatocellular carcinoma cell line HCC-9903 by fluorescence in situ hybridization(FISH)[J].Chinese Journal of General Surgery,2003,18(2):105-106. | |
| Authors: | LIU Jun WANG Zhan-min ZHENG Shu-fang |
| Institution: | LIU Jun,WANG Zhan-min,ZHENG Shu-fang,et al. Department of General Surgery,Qilu Hospital, Shandong University,Jinan 250012,China |
| Abstract: | Objective To study the X chromosome aberration in hepatocellular carcinoma cell line HCC-9903. Method The hepatocellular carc inoma cell line, HCC-9903, was detected by fluorescence in situ hybridization ( FISH) centromere DNA probes of X chromosome. Result Increased X chromosome copy number was observed in HC C-9903 cell line , four signals of hybridization was the major type. Conclusion Gain of X chromosome freq uently occurred in liver carcinoma. The relationship between gain of X chromosom e and liver carcinoma genesis needs further investigation. |
| Keywords: | Carcinoma hepatocellular X chromosome In situ hybridization fluorescence |
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