刺蒺藜总皂苷对雷公藤多苷诱导的急性肝损伤小鼠肝组织Caspase-3表达的影响

目的：探讨刺蒺藜总皂苷对急性药物性肝损伤的防治作用及其机理。方法：60只小鼠随机分为模型组，刺蒺藜总皂苷低、中、高剂量组，甘利欣对照纽和空白组，分别以相应剂量药物灌胃7d，然后以雷公藤多苷灌胃1次造模，观察小鼠血清AIJT、AST水平变化及肝组织Caspase-3表达情况。结果：刺蒺藜总皂苷可有效降低小鼠血清ATJT、AST水平，与模型组比较，有显著统计学意义（P〈0．05），并使肝组织Caspase-3表达降低，尤以高、中剂量组效果最佳。结论：刺蒺藜总皂苷对雷公藤多苷诱导的急性肝损伤具有较好保护作用，其机理与降低Caspase-3表达、抑制肝细胞凋亡有关。

Effect of Total Saponins from Tribulus Terrestris on Caspase - 3 Expression of Liver Tissues in Mice with Acute Liver Injury Induced by Tripterygium G!ycosides

Objective ：To explore the preventive and therapeutic effect and mechanisms of total saponins from tribulus terrestris（TSTT） on acute drug-induced liver injury. Methods ：60 mice were randomly divided into model group,low-dosage, medium-dosage and high -dosage group of TSTT, Ganlixin group and control group. Each group was respectively perfused with corresponding drug for 7 days and then tripterygium glycosides were perfused once to make models. The levels of ALT and AST in the serum were respectively measured and Caspase-3 expression of liver tissue was observed. Results ：The levels of ALT and AST in the serum of each TSTI＇ group reduced obvi- ously. Compared with model group ,the differences were statistically significant（ P 〈 0.05 ）. Meanwhile ,TSTI＇ could reduce Caspase-3 expression of liver tissue. The effects of high-dosage group and medium-dosage group were especially conspicuous. Conelusion：TSTr has better protective effect on acute liver injury induced by tripterygium glycosides, the mechanism of which is mainly related with reducing Caspase-3 expression and inhibiting apoptosis of the liver cells.