槟榔对豚鼠胃平滑肌的作用及机制探讨

目的：观察槟榔对豚鼠离体胃平滑肌肌条的作用,探讨其可能的效应机制。方法：制备豚鼠离体平滑肌肌条,以生理盐水为空白对照组,观察不同浓度槟榔对平滑肌肌条的效应（以Ach标化）,并比较加入阻断剂后槟榔作用效应的变化。结果：①不同浓度槟榔均能增高豚鼠离体胃平滑肌条的收缩力,增大收缩振幅及收缩波曲线下面积,与生理盐水对照组比较有显著增高（P〈0.01）。②阻断剂阿托品及维拉帕米可部分阻断槟榔的收缩效应,降低其作用后收缩振幅（P〈0.05）。结论：槟榔对豚鼠离体胃平滑肌条的收缩活动有明显兴奋作用,且呈剂量正相关性,M受体阻断剂阿托品和Ca2＋阻断剂维拉帕米可部分阻断这种效应,槟榔对豚鼠离体平滑肌肌条的收缩效应部分介导于M-胆碱能受体,L型电压依赖性Ca2＋通道。

Exploration of the Effect and Mechanism of Areca Seed on the Gastric Smooth Muscle of Guinea Pig

Objective：To observe the effect of Areca Seed（AS） on the isolated smooth muscle strips of guinea pig stomach in vitro and to explore the possible mechanism.Methods：Gastric smooth muscle strips of guinea pig were prepared.With saline solution as the blank control group,the effects of different concentrations of AS on the smooth muscle strips（all data are standardized by Ach） were observed and the effects of changes after adding the antagonist were analyzed.Results：Different concentrations of AS could increase the contractility of gastric smooth muscle strips of guinea pig and enlarge the contraction amplitude and contraction wave area under the curve.Compared with the saline solution control group,it were significantly higher（P〈0.01）.Antagonist atropine and verapamil could partially block the contraction effects of AS and reduce its role in contraction amplitude（P〈0.05）.Conclusion：The results indicate that AS has significant stimulant action on contractile activities of isolated gastric muscle strips in Guinea pig and this effect is positively correlated with the doses.The contraction effect of AS on the isolated smooth muscle strips of guinea pig stomach in vitro is mediated by the M-cholinergic receptors and L-type voltage-dependent Ca2＋ channels.