蜂毒素抑制骨肉瘤血管生成拟态与影响肿瘤细胞增殖、调亡的关系

目的：探讨蜂毒素抑制骨肉瘤裸鼠移植瘤的作用及机制。方法：建立裸鼠骨肉瘤原位移植瘤模型,裸鼠18只,随机分3组：生理盐水组,蜂毒素组,顺铂组。观察各组裸鼠骨肉瘤体积和瘤体质量抑制率;应用免疫组化CD34与PAS双重染色法检测各组裸鼠瘤体血管生成拟态密度;免疫组织化学法检测增值细胞核抗原（PCNA）蛋白表达;TUNEL法检测肿瘤细胞凋亡;运用相关性分析法研究蜂毒素抑制骨肉瘤血管生成拟态与影响肿瘤细胞增殖、凋亡的关系。结果：蜂毒素组瘤体积和瘤体质量抑制率分别为42.98%、39.03%,蜂毒素能明显抑制CD34与PAS双重染色法标记的肿瘤血管生成拟态密度,能明显抑制肿瘤细胞增殖、促进细胞凋亡,且蜂毒素抑制肿瘤血管生成拟态密度与肿瘤细胞增殖呈正相关、与细胞凋亡呈负相关。结论：蜂毒素具有抑制骨肉瘤裸鼠移植瘤生长的作用,其作用机制可能与其能够抑制肿瘤血管生成拟态、诱导肿瘤细胞凋亡、抑制细胞增殖有关

Experimental Study of the Relation of Inhibiting Vasculogenic Mimicry and Inducing Cell Apoptosis of Melittin on Osteosarcoa Xenotransplanted Models of Nude Mice

Objective：To study the antitumor effects and mechanism of Melittin on osteosarcoma xenotransplanted models of nude mice.Methods ：Xenotransplanted models of nude mice osteosarcoma cell UMR-106 in the laevo-hind tibia of nude mice were established.Inoculated mice were randomly divided into normal saline group,positive control group,Melittin group.All the nude mice were sacrificed after treatment,the size and weight of tumor were measured and the tumor volumes and the inhibition rates of tumor were calculated,Expression of PCNA was deteced by immunohistochemical method.The VM density in tumor tissues was detected by CD34 and PAS double staining in vivo.TUNEL semi-quantitative assay was used to study the melittin-induced apoptosis in OS cell line.To study mainly the relation of inhibiting vasculogenic mimicry and inducing cell apoptosis.Results ：Inhibiting rate ofmelittin in tumor capacity and weight was respectively 42.98%,39.03%.The mice treated with Melittin groups showed significantly smaller volume and weight of tumor than those of NS group after treatment.The VM density and PCNA of the Melittin group were significantly lower than those of the NS group.After treated by melittin,the apoptotic index detected by TUNEL assay increased remarkably.the relation of inhibiting vasculogenic mimicry and inducing cell apoptosis of Melittin is positive correlation,the relation of inhibiting vasculogenic mimicry and cell multiplication of Melittin is negative correlation.Conclusion ：The mechanism of the inhibition of the implanted tumor growth with Melittin treatment may be associated with the inhibition of vasculogenic mimicry in transplanted tumor.Furthermore,Melittin can induce cell apoptosis and inhibit cell multiplication