| HPLC测定苦杏仁不同炮制方法中苦杏仁苷的含量 |
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| 引用本文： | 尚严,李进,刘伟,崔永霞. HPLC测定苦杏仁不同炮制方法中苦杏仁苷的含量[J]. 河南中医药学刊, 2011, 0(7): 831-832 |
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| 作者姓名： | 尚严 李进 刘伟 崔永霞 |
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| 作者单位： | [1]沁阳市人民医院,河南沁阳454550 [2]河南中医学院分析测试中心,河南郑州450008 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目（编号：2009A360010） |
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| 摘 要： | 目的：建立反相高效液相色谱测定苦杏仁中苦杏仁苷含量的方法。方法：采用HPLC法,C18柱,流动相：甲醇-水（25∶75）;流速为1.0 mL/min;柱温30℃;检测波长210 nm。结果：苦杏仁苷在0.019 8-0.198 0 g/L范围内呈良好线性关系,r=1,平均回收率为97.68%（RSD=0.53%）。结论：该方法准确、可靠、专属性强,重复性好,可有效的控制苦杏仁的质量。
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| 关 键 词： | HPLC 苦杏仁 苦杏仁苷 含量测定 |
Determination of Different Processing Methods Amygdalin in Semen Armeniacae Amarum by HPLC |
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| Shang Yan,Li Jin,Liu Wei,Cui Yong-xia. Determination of Different Processing Methods Amygdalin in Semen Armeniacae Amarum by HPLC[J]. Journal of Henan college of Traditional Chinese Medicine, 2011, 0(7): 831-832 |
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| Authors: | Shang Yan Li Jin Liu Wei Cui Yong-xia |
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| Affiliation: | 2The People＇s Hospital of Qinyang,Qinyang,Henan,China 454550Center of Analysis and Measurement of Henan College of TCM,Zhengzhou,Henan,China 450008 |
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| Abstract: | Objective：To establish an HPLC method for content determination of amygdalin in Semen Armeniacae Amarum.Methods：The scopoletin was performed on a column C18;Themobile phase was methanol-water（25：75）;the flow rate was 1.0 mL/min;the column temperature：30 ℃;the detective wavelength was 210 nm.Result：The linear range of scopoletin was from 0.019 8 mg/mL to 0.198 0 mg/mL.The average recovery was 97.68%（RSD=0.53%）.Conclusion：The method is accurate,reliable,strong specialization and good repeatability.The paper can effectively for the quality Semen meniacae Amarum. |
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| Keywords: | HPLC amygdala amara amygdalin assaying |
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