对乙酰氨基酚对早产幼鼠模型血流动力学、左心室功能和血浆脑钠肽水平的影响

目的：探讨对乙酰氨基酚对早产幼鼠血流动力学、左心室功能和血浆脑钠肽（BNP）水平的影响，阐明其治疗早产儿动脉导管未闭（PDA）的作用机制。方法：孕鼠腹腔注射脂多糖（LPS）制备早产幼鼠模型，早产幼鼠分为模型对照组（n=18）和给药组（n=19），另选10只足胎龄幼鼠作为空白对照组。空白对照组幼鼠不做任何处理，模型对照组幼鼠不给药，给药组幼鼠连续3 d给予对乙酰氨基酚。彩色多普勒超声检测各组幼鼠血流动力学指标，包括左室射血分数（LVEF）、左心室舒张末内径（LVEDd）、左心室收缩末内径（LVESd）、短轴缩短率（FS）、左室收缩末期容积（ESV）和左室舒张末期容积（EDV），测定各组幼鼠血浆BNP水平。结果：模型对照组和给药组幼鼠体质量均低于空白对照组（P<0.05）。给予对乙酰氨基酚3 d后，与模型对照组比较，给药组幼鼠体质量增加（P<0.05）。与模型对照组比较，给药组幼鼠LVEF、FS和EDV升高（P<0.05），而LVEDd、LVESd和ESV降低（P<0.05）。与空白对照组比较，给药组幼鼠LVEF和FS降低（P<0.05），LVEDd、LVESd、EDV和ESV升高，但组间比较差异无统计学意义（P>0.05）。与空白对照组比较，模型对照组幼鼠血浆BNP水平明显升高（P=0.004）；与模型对照组比较，给药组幼鼠血浆BNP水平明显降低（P=0.009）。结论：对乙酰氨基酚可通过改善血流动力学指标降低血浆BNP水平，从而对幼鼠左心室功能起保护作用。

Effects of acetaminophen on hemodynamics,left ventricular function and plasma level of brain natriuretic peptide of premature young rats

Objective: To investigate the effects of acetaminophen on the hemodynamics, left ventricular function and plasma level of brain natriuretic peptide(BNP) in the premature young rats,and to clarify its mechanism in the treatment of patent ductus arteriosus(PDA) in the premature infants.Methods: The pregnant rats were injected intraperitoneally with lipopolysaccharide(LPS) to prepare the premature young rat models. The premature young rats were divided into model control group (n=18)and administration group (n=19);another 10 young rats with normal gestratonal age were selected and used as blank control group. The young rats in blank control group didn't receive any treatment, the young rats in model control group were not given any drug, and the premature young rats in administration group were continunously administrated with acetaminophen for 3 d. Color Doppler ultrasonography was used to detect the hemodynamic parameters of the young rats in various groups, including left ventricular ejection fraction (LVEF), left ventricular end diastolic diameter (LVEDd), left ventricular end systolic diameter (LVESd), fractional shortening (FS), left ventricular end-systolic volume (ESV), and left ventricular end-diastolic volume (EDV).The plasma BNP levels of young rats were detected.Results: The body weights of the young rats in administration group and model control group were lower than that in blank control group (P<0.05). After administration of acetaminophen for 3 d, compared with model control group, the body weight of the young rats in administration group was increased (P<0.05). Compared with model control group,the LVEF, FS and EDV of the young rats in administration group were increased (P<0.05),and the LVEDd, LVESd and ESV of the young rats were decreased(P<0.05). Compared with blank control group, the LVEF and FS of the young rats in administration group were decreased (P<0.05); the LVEDd, LVESd, EDV and ESV of the young rats were increased, but the differences were not statistically significant (P>0.05).Compared with blank control group, the plasma BNP level of the young rats in model control group was significantly increased(P=0.004);compared with model control group, the plasma BNP level of the young rats in administration group was significantly decreased(P=0.009).Conclusion: Acetaminophen can protect the left ventricular function of the young rats by improving the hemodynamic indicators and reducing the plasma BNP level.