糖尿病模型大鼠烫伤创面皮肤组织P物质和Bcl-2表达与龙血竭的干预*

背景：研究证明龙血竭有促进创伤愈合、抗血小板聚集、抗炎镇痛、抗菌抗氧化等生物活性。 目的：验证龙血竭在糖尿病大鼠烫伤皮肤组织愈合过程中对P物质和Bcl-2表达的影响。 方法：Wistar大鼠112只,随机分为4组。龙血竭组、磺胺嘧啶银(SD-Ag)组、糖尿病对照组和正常对照组。其中前3组为糖尿病深Ⅱ度烫伤模型,正常对照组为正常深Ⅱ度烫伤模型。 结果与结论：烫伤后第7天开始创面愈合率龙血竭组、正常对照组高于SD-Ag组和糖尿病对照组(P < 0.05),第21天龙血竭组创面愈合率明显高于SD-Ag组和糖尿病对照组(P < 0.05),正常对照组最高。烫伤后第15天正常对照组P物质阳性表达水平最高,峰值显著高于其他3组,以后逐步减弱。龙血竭组、SD-Ag组和糖尿病对照组于烫伤后第21天阳性表达最强,且龙血竭组表达明显高于SD-Ag组和糖尿病对照组(P < 0.05)。烫伤后第3天开始正常对照组Bcl-2阳性表达水平明显高于其他3组;于第15天各组阳性表达水平皆达到峰值,龙血竭组最高,明显高于SD-Ag组和糖尿病对照组(P < 0.05),与正常对照组比较差异无显著性意义(P > 0.05)。说明龙血竭能通过参与调控P物质和Bcl-2的表达,有效地促进糖尿病烫伤创面的愈合。

Effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats

BACKGROUND: Previous studies have demonstrated that dragon’s blood can promote the wound healing as well as have characteristics of anti-platelet aggregation, anti-inflammatory analgesic, anti-bacterial and antioxidant and other biological activity. OBJECTIVE: To identify the effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats. METHODS: A total of 112 Wistar rats were randomly divided into four groups: dragon’s blood group, silver sulfadiazine (SD-Ag) group, diabetic control group and normal control group. Diabetic deep Ⅱ degree burn models were established in dragon’s blood group, SD-Ag group and diabetic control group, and the normal deep Ⅱ degree burn models were established in normal control group. RESULTS AND CONCLUSION: On the 7th day post scald, the wound healing rates of dragon’s blood group and normal control group were higher than those of SD-Ag group and diabetic control group (P < 0.05). After 21 days, the wound healing rate of dragon’s blood group was significantly higher than that of SD-Ag group and diabetic control group (P < 0.05) and highest in normal control group. After 15 days, the positive expression level of substance P reached a peak in normal control group and was higher than other three groups, and then decreased gradually. The expression was most positive in dragon’s blood group, SD-Ag group and diabetic control group at the 21th day post scald, and which was significantly higher in dragon’s blood group (P < 0.05). On the 3rd day after scald, the expression level of Bcl-2 in the normal control group was significantly higher than that in other groups. From the 15th day, the expression of Bcl-2 was gradually increased in each group and reached to peak, and highest in dragon’s blood group (P < 0.05), but there was no significant difference between dragon’s blood group and normal control group (P > 0.05). The results demonstrate that dragon's blood can promote the healing of diabetic burn wounds significantly by regulating the expression of substance P and Bcl-2.