| 胸椎黄韧带骨化症与骨形态发生蛋白4基因单核苷酸多态性的关联 |
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| 引用本文： | 赵伟光,刘振武,刘 利,谢延平,江丽强,林 欣. 胸椎黄韧带骨化症与骨形态发生蛋白4基因单核苷酸多态性的关联[J]. 中国组织工程研究, 2012, 16(24): 4376-4380. DOI: 10.3969/j.issn.1673-8225.2012.24.002 |
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| 作者姓名： | 赵伟光 刘振武 刘 利 谢延平 江丽强 林 欣 |
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| 作者单位： | 1邯郸市中心医院骨一科,河北省邯郸市 056001; 2首都医科大学附属北京天坛医院骨科,北京市 100050 |
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| 基金项目： | 国家自然科学基金(30872599);北京市自然科学基金(7092028) |
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| 摘 要： | 背景：骨形态发生蛋白4基因与胸椎黄韧带骨化症的相关性研究目前较少。目的：测观察骨形态发生蛋白4的2个单核苷酸多态性位点rs17563和rs2855532与胸椎黄韧带骨化症的关联。方法：胸椎黄韧带骨化症患者和正常对照者各40例,收集受试者的周围静脉血提取DNA,PCR法进行目的片段骨形态发生蛋白4的单核酸多态性位点rs17563和rs2855532的扩增并测序。结果与结论：黄韧带骨化症组中rs17563和rs2855532位点带“T”基因型及等位基因型频率明显高于对照组(P < 0.05)。证实,骨形态发生蛋白4上的2个单核酸多态性位点rs17563和rs2855532等位基因型突变与胸椎黄韧带骨化症的发生有关。
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| 关 键 词： | 黄韧带骨化症 胸椎 骨形态发生蛋白4 单核苷酸多态性 聚合酶链反应 病例-对照研究 组织构建 |
| 收稿时间： | 2012-04-01 |
Bone morphogenetic protein-4 gene polymorphism associated with ossification of the thoracic ligamentum flavum |
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| Zhao Wei-guang,Liu Zhen-wu,Liu Li,Xie Yan-ping,Jiang Li-qiang,Lin Xin. Bone morphogenetic protein-4 gene polymorphism associated with ossification of the thoracic ligamentum flavum[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(24): 4376-4380. DOI: 10.3969/j.issn.1673-8225.2012.24.002 |
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| Authors: | Zhao Wei-guang Liu Zhen-wu Liu Li Xie Yan-ping Jiang Li-qiang Lin Xin |
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| Affiliation: | 1Department of Orthopedics, Handan Central Hospital, Handan 056001, Hebei Province, China;
2Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China |
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| Abstract: | BACKGROUND:There are few studies addressing the association of bone morphogenetic protein-4 gene and ossification of the thoracic ligamentum flavum (OLF).OBJECTIVE:To explore whether two single nucleotide polymorphisms of the bone morphogenetic protein-4 were related to the morbidity and severity of OLF.METHODS:The venous blood samples of 40 OLF patients and 40 non-OLF controls were collected. All experimental participants were matched in age-sex composition. The DNA extracted from the peripheral venous blood was amplified and sequenced at two sites of rs17563 and rs2855532 by using PCR.RESULTS AND CONCLUSION:The frequency of “T” genotype and allelotype at rs17563 and rs2855532 in the OLF patients was higher than that in the non-OLF patients (P < 0.05). The two single nucleotide polymorphisms (rs17563 and rs2855532) can increase the incidence of OLF. |
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