人骨髓间充质干细胞培养上清与肝星状细胞相关酶的分泌 |
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作者姓名: | 王 珊 张立婷 陈 红 |
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作者单位: | 兰州大学第一医院,甘肃省兰州市 730030 |
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摘 要: | 背景:研究证实骨髓间充质干细胞移植治疗能改善甚至逆转肝纤维化,但其具体机制尚不清楚。
目的:检测人骨髓间充质干细胞培养上清对肝星状细胞中基质金属蛋白酶2及组织抑制基质金属蛋白酶1表达的影响。
方法:采用密度梯度离心法分离人骨髓间充质干细胞,收集原代培养7 d的骨髓间充质干细胞培养上清,加入肝星状细胞中培养作为实验组,并设置单独肝星状细胞组、单独骨髓间充质干细胞组为对照。培养24,48 h后,检测各组细胞上清中基质金属蛋白酶2蛋白与组织抑制基质金属蛋白酶1蛋白的表达。
结果与结论:与单独肝星状细胞组、单独骨髓间充质干细胞组比较,实验组培养24,48 h后肝星状细胞基质金属蛋白酶2及组织抑制基质金属蛋白酶1表达减少(P < 0.05或P < 0.01)。表明骨髓间充质干细胞培养上清抑制肝星状细胞中基质金属蛋白酶2、组织抑制基质金属蛋白酶1的表达。
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关 键 词: | 肝星状细胞 骨髓间充质干细胞 肝纤维化 基质金属蛋白酶2 组织抑制基质金属蛋白酶1 |
收稿时间: | 2012-01-03 |
Effect of human bone marrow mesenchymal stem cells culture supernatant on related enzyme expression in hepatic stellate cells |
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Authors: | Wang Shan Zhang Li-ting Chen Hong |
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Institution: | The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China |
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Abstract: | BACKGROUND:The studies have proved that bone marrow mesenchymal stem cells transplantation therapy can improve or even reverse the liver fibrosis, but its mechanism is still unclear.
OBJECTIVE:To detect the effect of human bone marrow mesenchymal stem cells culture supernatant on the expression of matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 1 in hepatic stellate cells.
METHODS:The density gradient centrifugation was used to isolate human bone marrow mesenchymal stem cells and collected the culture supernatant of human bone marrow mesenchymal stem cells after cultured for 7 days, the human bone marrow mesenchymal stem cells culture supernatant added with the hepatic stellate cells was considered as experimental group, a simple hepatic stellate cells group and simple human bone marrow mesenchymal stem cells group were set up as control groups. The expressions of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the culture supernatant of three groups were detected after cultured for 24 and 48 hours.
RESULTS AND CONCLUSION:The expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the experimental group was decreased when compared with simple hepatic stellate cells group and simple human bone marnow mesenchymal stem cells group (P < 0.05 or P < 0.01). The results showed that bone marrow mesenchymal stem cells culture supernatant can inhibit the expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in hepatic stellate cells. |
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