内关穴位埋针心肌缺血损伤小型猪血管生长功能性基因的表达谱

背景：内关针刺可上调相关血管生长因子的表达，但血管新生相关生长因子较多，针对部分因子研究有局限性。 目的：观察心肌缺血损伤小型猪血管生长功能性基因表达谱特征及内关穴位埋针的干预效应。 方法：将32只小型猪随机分为4组，模型组采用左冠状动脉前降支结扎法建立小型猪心肌缺血模型，内关组和膈俞组分别在造模基础上进行内关、膈俞穴埋针治疗，假手术组穿线不结扎。埋针7 d后取各组小型猪的缺血心肌组织进行实验。 结果与结论：应用Q Series血管生长功能基因组表达谱芯片共检测到96个与血管生成功能密切相关的基因。与模型组比较，内关组显著上调基因17个，下调基因3个，膈俞组显著上调基因14个，下调基因2个。就其基因功能分类而言，内关组、膈俞组与模型组比较差异表达基因各有其自身特点，但特异的促进因子和抑制因子、血小板衍生生长因子及受体、成纤维细胞生长因子及受体、生长因子及受体以及其他细胞生长因子，细胞因子和化学增活素、基质蛋白酶及抑制剂以及其他相关基因是各组共有的，其中以血管生长因子及受体最多。说明心肌缺血以及穴位埋针后的干预均与血管生长因子差异表达有关，但内关埋针与膈俞埋针作用不完全相同。

Gene-expression profiles about myocardial ischemia injury miniature pig’s growth function and intervention effects of needle-embedding therapy at Neiguan

BACKGROUND:Acupuncture at Neiguan (PC6) can up-regulate relative expressions of vascular growth factors. Nonetheless, there are plenty of growth factors related to angiogenesis, and researches have limitations if just focusing on a part of these factors. OBJECTIVE:To observe characteristics on gene-expression profiles about myocardial ischemia injury miniature pig’s growth function and intervention effects of needle-embedding therapy at Neiguan. METHODS:Thirty-two miniature pigs were randomized into four groups. Afterwards, ligation of the left anterior descending coronary artery was performed to establish myocardial ischemia models. Meanwhile, pigs in sham operation group only underwent threading without ligation, while needle-embedding therapy at Neiguan and Geshu (BL17) was conducted in Neiguan group and Geshu group, respectively. Then, ischemic myocardial tissue was extracted for experiments after 7 days needle-embedding therapy. RESULTS AND CONCLUSION:A total of 96 genes were detected that were closely related to the function of angiogenesis by using Q Series Angiogenesis’ functional genomics expression profiles. Compared with the model group, there were 17 significantly up-regulated genes and 3 down-regulated genes in the Neiguan group; and 14 significantly up-regulated genes and 2 down-regulated genes in the Geshu group. As to classifications of gene function, differentially expressed genes had their own features when comparing differences expressions among Neiguan group, Geshu group and model group. Nevertheless, distinctive promoting factor and inhibitor, platelet derived growth factor and receptor, fibroblast growth factor and receptor, growth factor and receptor, other cell growth factors, cytokine and chemokines active element, matrix metalloproteinase and inhibitor, as well as other relative genes were shared between groups; among them, angiogenesis factors and receptors were the most. It indicates that intervention for myocardial ischemia and needle-embedding therapy are related to angiogenesis factors, but the action mechanism of needle-embedding therapy at Neiguan is different from that at Geshu.