骨髓间充质干细胞移植脊髓损伤小鼠运动功能的恢复 |
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作者姓名: | 刘声亮 范业文 李 岩 张宏男 薛萌萌 陈明伟 孔 建 刘 欣 崔开宇 彭 艳 王 铎 刘京晶 葛小平 王 勇 吴树亮 |
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作者单位: | 哈尔滨医科大学,1附属医院,3解剖学教研室,黑龙江省哈尔滨市 150081;2黑龙江中医药大学,黑龙江省哈尔滨市 150040;4解放军第二一一医院骨一科,黑龙江省哈尔滨市 150080 |
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摘 要: | 背景:研究证实,移植的骨髓间充质干细胞可被定向诱导分化为神经细胞,重建神经环路,促进轴突再生,恢复脊髓功能。
目的:进一步验证骨髓间充质干细胞在脊髓损伤修复中的作用。
方法:C57BL/6小鼠40只随机分为4组,假手术组不打击脊髓;其余小鼠采用重物撞击法建立脊髓损伤模型。损伤后的第7天,治疗组用微量注射器,经眶静脉丛注入骨髓间充质干细胞悬液;对照组注入等量 DMEM培养基;模型组不做处理。通过苏木精-伊红染色法判断脊髓损伤程度。通过免疫细胞化学法鉴定骨髓间充质干细胞分化形成的神经细胞。通过荧光显微镜观察移植细胞生长状态;通过改良Tarlov评分法评价小鼠运动功能恢复程度。
结果与结论:骨髓间充质干细胞诱导分化7 d后的细胞呈NF和神经胶质纤维酸性蛋白阳性表达。模型组小鼠双下肢呈瘫痪状态,假手术组行动正常(P < 0.01)。细胞移植后2周,治疗组小鼠运动功能缺失症状逐渐恢复,对照组小鼠恢复不明显 (P < 0.05);细胞移植4周后,细胞移植组小鼠Tarlov评分与假手术组相比差异无显著性意义(P > 0.05)。说明骨髓间充质干细胞移植可提高脊髓损伤小鼠的运动能力。
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关 键 词: | 骨髓间充质干细胞 分化 细胞移植 脊髓损伤 Tarlov评分 小鼠 运动功能 干细胞 |
收稿时间: | 2012-03-15 |
Bone marrow mesenchymal stem cells transplantation promotes the motor function recovery after spinal cord injury |
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Authors: | Liu Sheng-liang Fan Ye-wen Li Yan Zhang Hong-nan Xue Meng-meng Chen Ming-wei Kong Jian Liu Xin Cui Kai-yu Peng Yan Wang Duo Liu Jing-jing Ge Xiao-ping Wang Yong Wu Shu-lian |
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Institution: | 1Affiliated Hospital of Harbin Medical University, Harbin 150081, Heilongjiang Province, China; 2Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China; 3Department of Anatomy, Harbin Medical University, Harbin 150081, Heilongjiang Province, China; 4First Department of Orthopedics, the 211 Hospital of Chinese PLA, Harbin 150080, Heilongjiang Province, China |
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Abstract: | BACKGROUND:The studies have confirmed that the bone marrow mesenchymal stem cells transplantation can be induced to differentiate into neural cells, reconstruct neuronal circuits, and promote axonal regeneration so as to recover of spinal cord function.
OBJECTIVE:To explore the repair effect of bone marrow mesenchymal stem cells on spinal cord injury.
METHODS:Forty C57BL/6 mice were divided into four groups, mice in the sham-operation group did not combat the spinal cord, and mice in the other three groups were used to establish the spinal cord injury model by weight impact method. At 7 days after injury, bone marrow mesenchymal stem cell suspension was injected in treatment group via orbital venous plexus by microinjector; mice in the control group were injected with DEME culture medium in the same dose; mice in the model group were not treated. The extent of spinal cord injury was detected by hematoxylin-eosin staining. Nerve cells differentiated from bone marrow mesenchymal stem cells were identified by immunocytochemistry staining. The growth state of transplanted cells was observed by fluorescent microscope. Motor functional recovery was evaluated by modified Tarlov score.
RESULTS AND CONCLUSION:After the bone marrow mesenchymal stem cells were induced to differentiate for 7 days, positive expression of nuclear factor and glial fibrillary acidic protein could be seen in the cells. Both lower extremities of the mice in the model group were paralyzed, and the mice in the sham-operation group had normal action (P < 0.01). At 2 weeks after transplantation, the symptoms of motor functional deficits in the treatment group was gradually restored, and the recovery in the sham-operation group was not obvious (P < 0.05); at 4 weeks after transplantation, there was no significant difference of the Tarlov score between treatment group and sham-operation group (P > 0. 05). Bone marrow mesenchymal stem cells transplantation can promote the motor functional recovery after spinal cord injury. |
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