异种生物衍生骨支架材料的制备及性能

背景：异种骨来源丰富，价格低廉，处理相对简单容易，处理后骨支架保留原有骨的微结构，具有良好的促成骨、骨传导及骨诱导活性。 目的：检测自制生物衍生骨支架材料的理化性质及体外细胞相容性。 方法：通过脱蛋白、脱脂、脱钙，深低温冻存制备猪源性松质骨支架材料。组织学检测松质骨处理前后的变化，扫描电镜观察材料结构及计算孔隙直径，采用液体置换法检测支架材料的孔隙率，体外降解速度，能谱分析及体外复合兔骨髓间充质干细胞的细胞相容性。 结果与结论：处理后的松质骨支架材料具有三维多孔结构，孔隙直径150.8-306.7 μm，孔隙率84.5%-89.7%。材料在前6周降解速度稍慢，6周后材料降解率曲线基本呈线性且降解速度明显加快，10周时材料接近完全降解，降解率达92.8%。松质骨支架材料孔隙大小适合骨髓间充质干细胞的黏附和增殖。表明生物衍生骨支架材料性能良好，细胞相容性良好，适用于构建组织工程骨。

Preparation and properties of xenogeneic biological derived bone scaffold materials

BACKGROUND:Xenogeneic bone has the advantages of rich source, inexpensive and relatively simple and easy approach. Bone scaffold after processing can retain the micro-structure of the original bone. It has a good contribute to bone, bone conduction and bone-inducing activity. OBJECTIVE:To detect the physiochemical performance and cells compatibility in vitro of homemade biological derivative bone scaffold materials. METHODS:The pig cancellous scaffold materials were prepared through deproteinization, degrease, decalcification and cryopreservation. The cancellous scaffold materials before and after processed were detected by histological observation. Material structure and pore diameter was observed and calculated under scanning electron microscope. Scaffold materials porosity was calculated by using liquid replacement method, and the degradation speed of scaffolds in vitro was tested. Materials mainly elements were detected by the energy spectrum analysis and the compatibility of rabbit bone marrow mesenchymal stem cells in vitro. RESULTS AND CONCLUSION:Processed scaffolds had the characteristics of three-dimensional structure. The pore size was 150.8-306.7 μm and the porosity was 84.5%-89.7%. Scaffolds degradation was at a slow pace in the first 6 weeks. After the first 6 weeks, the scaffolds degradation rate curve presented linear curve and the degradation speed was increased significantly. The material was almost completely degraded at 10 weeks and the degradation rate arrived to 92.8%. The pore size of cancellous scaffold was appropriate for the proliferation and adhesion of bone mesenchymal stem cells, it indicated that the biological derivative bone scaffold materials had good performance and compatibility and suitable for building tissue engineering bone.