骨关节炎模型兔膝关节腔注射丹皮酚联合针刺足三里血清白细胞介素1β、肿瘤坏死因子α的变化

背景：针灸和西药关节腔注射对骨关节炎均有临床疗效,少见针灸联合中药关节腔注射治疗骨关节炎的报道。 目的：验证骨关节炎模型兔膝关节腔注射丹皮酚联合针刺足三里后血清白细胞介素1β、肿瘤坏死因子α水平的影响。 方法：实验分为正常组(不作处理)、模型组、中药组、针刺组、针药组5组。后4组采用膝关节腔注射木瓜蛋白酶的方法复制出家兔膝骨关节炎模型。模型组不作处理;中药组向兔右膝关节腔注射丹皮酚,1次/周,共5次;针刺组针刺兔双侧足三里穴,1次/d,共29次;针药组向兔右膝关节腔注射丹皮酚,1次/周,共5次,同时针刺兔双侧足三里穴,1次/d,共29次。于治疗后测定血清中白细胞介素1β、肿瘤坏死因子α的含量。 结果与结论：中药组、针刺组、针药组的白细胞介素1β及肿瘤坏死因子α水平均低于模型组(P < 0.05),高于正常组(P < 0.05);而针药组血清中白细胞介素1β、肿瘤坏死因子α的含量显著低于中药组、针刺组(P < 0.05)。提示膝关节腔注射丹皮酚联合针刺足三里可显著降低白细胞介素1β及肿瘤坏死因子α水平,抑制白细胞介素1β和肿瘤坏死因子α对关节软骨的破坏作用,延缓关节软骨的退变。 

Effect of intra-articular injection with paeonol and acupuncture at Zusanli on serum interleukin 1-beta and tumor necrosis factor alpha contents in a rabbit model of knee osteoarthritis

BACKGROUND: Acupuncture or intra-articular injection with western medicine both can effectively treat osteoarthritis, but the therapeutic effect of acupuncture and intra-articular injection with traditional Chinese drug treating osteoarthritis is rarely reported. OBJECTIVE: To explore the effect of intra-articular injection with paeonol and acupuncture at Zusanli (ST36) on interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in serum of rabbit’s knee osteoarthritis model. METHODS: Rabbit models of knee osteoarthritis were randomly divided into five groups: normal group (without treatment), model group, paeonol group, acupuncture group and paeonol+acupuncture group. The last four groups were made by intra-articular injection with papain. The model group was without treatment. The paeonol group was treated with the intra-articular injection with paeonol, once a week, a total of 5 times. The acupuncture group was treated with acupuncture of rabbit bilateral Zusanli, once a day and a total of 29 times. The paeonol+acupuncture group was treated with the intra-articular injection with paeonol, once time a week, a total of 5 times, and the acupuncture Zusanli was performed at the same time, once a day and a total of 29 times. The content of IL-1β and TNF-α in serum were measured after treatment. RESULTS AND CONCLUSION: The contents of IL-1β and TNF-α in paeonol group, acupuncture group and paeonol+acupuncture group were lower than those in model group (P < 0.05) but higher than those in normal group (P < 0.05). However, the contents of IL-1β and TNF-α in paeonol+acupuncture group were lower than those in paeonol group and acupuncture group (P < 0.05). It was considered that intra-articular injection with paeonol and acupuncture Zusanli could significantly degrade the content of IL-1β and TNF-α in serum and inhibit the damage effect of IL-1β and TNF-α on articular cartilage which could potentially delay the degeneration of joint cartilage.