骨髓间充质干细胞静脉移植脊髓损伤大鼠肿瘤坏死因子α及 白细胞介素1β的表达

背景：研究认为间充质干细胞的营养支持在脊髓损伤治疗中起了主要作用,其同损伤宿主神经组织间的相互作用可导致一些不利于损伤修复的炎症因子表达减少。 目的：观察大鼠骨髓间充质干细胞静脉注射移植对脊髓损伤后肿瘤坏死因子α、白细胞介素1β 表达的影响。 方法：运用改良Allen法制备大鼠T10脊髓外伤性截瘫模型,随机分为对照组和骨髓间充质干细胞移植组,设未损伤脊髓的假手术组做对照。骨髓间充质干细胞移植组、假手术组均接受大鼠骨髓间充质干细胞静脉注射移植,对照组静脉注射等量PBS。 结果与结论：对照组和骨髓间充质干细胞移植组损伤脊髓肿瘤坏死因子α、白细胞介素1β蛋白表达较假手术组有明显增加(P < 0.05);骨髓间充质干细胞移植组与对照组比较, 肿瘤坏死因子α、白细胞介素1β蛋白表达受到明显抑制(P < 0.05)。提示大鼠骨髓间充质干细胞静脉移植后能使损伤脊髓局部的肿瘤坏死因子α、白细胞介素1β表达程度降低。这可能是改变脊髓损伤区的微环境,减少脊髓继发性损伤,促进损伤大鼠运动功能恢复的机制之一。

Expression of tumor necrosis factor-alpha and interleukin 1 beta in spinal cord injury rats undergoing intravenous transplantation of bone marrow mesenchymal stem cells

BACKGROUND:Evidence exists that nutrition of bone marrow mesenchymal stem cells plays an important role in the treatment of spinal cord injury. The interaction between bone marrow mesenchymal stem cells and damaged host nerve tissue can reduce the expression of inflammatory cytokines which is conductive to injury recovery. OBJECTIVE:To explore the effect of intravenous transplantation of bone marrow mesenchymal stem cells on the expression of tumor necrosis factor-α and interleukin 1β after spinal cord injury in rats. METHODS:The T10 paraplegia model of rat spinal cord injury was prepared using modified Allen. The experiment was divided into control group and transplantation group. The sham operation group with no spinal cord injury was designated. The transplantation group and sham operation group were given intravenous administration of bone marrow mesenchymal stem cells, the same concentration of PBS was intravenously injected into the control group. RESULTS AND CONCLUSION:Expression of tumor necrosis factor-α and interleukin 1β protein in the transplantation group and control group was higher than that in the sham operation group (P < 0.05). Compared with the control group, the expression of tumor necrosis factor-α and interleukin 1β protein in the transplantation group was significantly inhibited (P < 0.05). Intravenous transplantation of bone marrow mesenchymal stem cells could reduce the expression of tumor necrosis factor-α and interleukin 1β in the injured area of spinal cord. Maybe it was one of the mechanisms to change the microenvironment of the injured area, decrease the secondary spinal cord injury and promote the recovery of motor function in spinal cord injury rats.