碱性成纤维细胞生长因子复合纳米羟基磷灰石/壳聚糖复合物修复兔桡骨缺损

背景：纳米羟基磷灰石/壳聚糖复合物具有良好的力学性能和生物相容性,可用于骨损伤的修复,是一种有应用前景的骨替代品。碱性成纤维细胞生长因子可促进组织修复,目的：观察碱性成纤维细胞生长因子复合纳米羟基磷灰石/壳聚糖复合物修复兔骨缺损的效果。方法：构建桡骨缺损兔模型,按植入材料的不同共分为3组,实验组植入纳米羟基磷灰石/壳聚糖复合物+碱性成纤维细胞生长因子,对照植入组纳米羟基磷灰石/壳聚糖复合物,空白组无任何材料植入。结果与结论：干预12周时,X射线片检查显示,实验组骨植入区新骨发生骨性融合,髓腔再通骨缺损已基本消失;苏木精-伊红染色结果显示,实验组出现成熟的板层骨、成熟的哈弗氏系统以及破骨细胞增生引起的骨质吸收区;以上结果均为实验组的修复效果优于对照组。证实,碱性成纤维细胞生长因子复合纳米羟基磷灰石/壳聚糖复合物可促进骨缺损修复,效果优于单独应用纳米羟基磷灰石/壳聚糖复合物。

Basic fibroblast growth factor combined with nano-hydroxyapatite/chitosan composites for repair of radial bone defects in rabbits

BACKGROUND:Nano-hydroxyapatite/chitosan composite has good mechanical properties and biocompatibility, which is suitable for repairing bone defects replacing homograft bone. Basic fibroblast growth factor can improve tissue repair.OBJECTIVE:To observe the effect of basic fibroblast growth factor combined with nano-hydroxyapatite/chitosan on radial bone defects in rabbits.METHODS:A total of 36 New Zealand adult healthy rabbits were collected to establish rabbit bone defect models, and then divided into three groups according to different implant materials: implantation of nano-hydroxyapatite/chitosan and basic fibroblast growth factor in experimental group, implantation of nano-hydroxyapatite/chitosan in control group, and no implant materials in blank group.RESULTS AND CONCLUSION:After 12 weeks of intervention, X-ray examination revealed bone fusion in the implanted area in the experimental group, and bone defects disappeared nearly. Hematoxylin-eosin staining showed that mature lamellar bone, mature Haversian system and bone resorption induced by the proliferation of osteoclasts were found in the experimental group. These results were the repair effects of the experimental group superior to the control group. It has been verified that basic fibroblast growth factor combined with nano-hydroxyapatite/chitosan has a better effect on repairing bone defects, superior to simple nano-hydroxyapatite/chitosan composite.