兔脂肪干细胞体外诱导分化为胰岛β样细胞的可行性 |
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作者姓名: | 杨 光 赵占志 朱新兵 胡 松 |
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作者单位: | 辽河油田中心医院,1普外科, 2麻醉科,辽宁省盘锦市 124000 |
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摘 要: | 背景:胚胎干细胞、胰腺干细胞、肝卵圆细胞、小肠上皮干细胞和骨髓间充质干细胞均可以在体外诱导分化为胰岛β样细胞,那么脂肪干细胞是否也可以呢?
目的:探讨兔脂肪干细胞向胰岛β样细胞分化的可行性。
方法:以常规方法从兔脂肪抽吸物中分离、培养脂肪干细胞;传2代后,用高浓度葡萄糖(25 mmol/L)培养液DMEM以及碱性成纤维生长因子和尼克酰胺诱导兔脂肪干细胞向胰岛β样细胞分化。
结果与结论:第2代兔脂肪干细胞经过高糖诱导后,细胞形成细胞团;并且形成了双硫腙染色阳性的细胞团;免疫细胞化学表明诱导后细胞内的细胞胰岛素染色阳性。说明兔脂肪干细胞在体外可以定向诱导分化为胰岛β样细胞,这种胰岛β样细胞具有表达、储存胰岛素的功能。
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关 键 词: | 胰岛β样细胞 脂肪干细胞 体外分化 碱性成纤维细胞生长因子 尼克酰胺 干细胞 |
收稿时间: | 2011-11-03 |
Differentiation of adipose-derived stem cells into islet β-like cells in vitro |
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Authors: | Yang Guang Zhao Zhan-zhi Zhu Xin-bing Hu Song |
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Institution: | 1Department of General Surgery;
2Department of Anesthesiology, the Central Hospital of Liaohe Oil Field, Panjin 124000, Liaoning Province, China |
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Abstract: | BACKGROUND:Islet β-like cells can be induce-differentiated by embryonic stem cells, pancreatic stem cells, hepatic oval cells, intestinal stem cells and bone marrow mesenchymal stem cells in vivo. Then whether adipose-derived stem cells can be differentiate into islet β-like cells remains pooly understod?
OBJECTIVE:To investigate the feasibility of adipose-derived stem cells to differentiate into islet β-like cells.
METHODS:Adipose-derived stem cells were isolated and cultured from rabbit liposuction, and after two generations passage, the adipose-derived stem cells were differentiated into islet β-like cells induced by high glucose, basic fibroblast growth factorand nicotamine in culture medium.
RESULTS AND CONCLUSION:After the passage 2 adipose-derived stem cells were induced by high glucose, the adipose-derived stem cells formed cell clusters and dithizone-stained cell clusters in flask were observed. Immunocytochemistry also confirmed that these cell clusters were positive for insulin. Adipose-derived stem cells can differentiate into islet β-cells in vitro. These islet β-cells can synthesize and store insulin. |
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