大鼠胚胎神经干细胞体外分化时间的特异性

背景：目前对于神经干细胞体外培养过程中神经干细胞自身分化特性的变化研究的较少。目的：观察大鼠胚胎神经干细胞体外分化的时间特异性。方法：利用无血清悬浮培养的方法分离培养大鼠胚胎皮质神经干细胞,取不同培养时间的神经干细胞进行诱导分化,检测其分化特性的改变。结果与结论：体外培养的神经干细胞早期分化以神经元为主,随着培养时间的增加,分化成神经元的比例下降,神经胶质细胞的比例增加,而神经干细胞分化成少突胶质细胞的潜能与培养时间无明显相关。提示体外培养的神经干细胞其分化成神经元的潜能随着培养时间的增加而降低,而分化成神经胶质细胞的潜能随培养时间的增加而增加,神经干细胞分化为少突胶质细胞的潜能无时间相关性。

Temporal specificity of differentiation of rat embryonic neural stem cells cultured in vitro

BACKGROUND:There have been few studies describing the characteristics of self differentiation of neural stem cells cultured in vitro.OBJECTIVE:To investigate the temporal specificity of differentiation of rat embryonic neural stem cells cultured in vitro.METHODS:Neural stem cells were isolated from rat embryonic cerebral cortex and cultured in serum-free medium. Neural stem cells cultured for different time were induced to differentiate and the change of differentiation properties was detected.RESULTS AND CONCLUSION:Neural stem cells cultured in vitro were induced to differentiate predominately into neurons at early phase. As the increase of incubation time, the proportion of neural stem cells differentiating into neurons was decreased, and the proportion of neural stem cells differentiating into astrocytes was increased. However, there was no obvious correlation between the differentiation potential of neural stem cells into oligodendrocytes and incubation time. These findings suggest that as the increase of incubation time, the differentiation potential of neural stem cells into neurons was reduced and the differentiation potential of neural stem cells into astrocytes was enhanced; there was no obvious correlation between the differentiation potential of neural stem cells into oligodendrocytes and incubation time.