磷酸三钙陶瓷材料形状对大鼠体内成血管化的影响

背景：组织工程人工骨支架材料的大体和微观结构可加速血管化进程。 目的：观察不同形状β-磷酸三钙陶瓷骨在体内的血管化程度，探索载体材料形状对体内血管化的影响。 方法：将柱状和管状两种β-磷酸三钙陶瓷骨材料分别植入SD大鼠两侧腰背筋膜下。 结果与结论：①同位素扫描结果：术后第3，6，12周，管状β-磷酸三钙陶瓷骨材料放射性核素骨显影放射性计数明显高于柱状β-磷酸三钙陶瓷骨材料(P < 0.05)，并且随着时间的延长，两组放射性核素骨显影放射性计数均呈上升趋势。②扫描电镜观察结果：第3周时，材料外形保持良好，宿主纤维组织和血管由材料外周向内生长；术后6周时，管状材料内小血管增生活跃，且分布比较均匀，柱状材料血管增生主要集中在外周部分；术后12周时，两组材料血管化程度更高，管状材料外周及中心部位均可见较为成熟的纤维组织和丰富血管网，但柱状材料纤维组织和血管主要集中在外周部分，中轴部分较少。表明管状三维结构β-磷酸三钙陶瓷骨材料较柱状材料更利于体内血管化。

Effects of different shapes of beta-tricalcium phosphate ceramics on vascularization in vivo of rats

BACKGROUND:The gross and microscopic structure of artificial bone scaffold materials for tissue engineering can accelerate the process of vascularization. OBJECTIVE:To investigate the degree of vascularization in vivo by using different shapes of β-tricalcium phosphate ceramics, to explore the degree of blood vessels, and to explore the effect of the scaffold materials shapes on vascularization in vivo. METHODS:Columnar and tubular β-tricalcium phosphate ceramics were implanted in the bilateral back fascia of SD rats. RESULTS AND CONCLUSION:Radioisotope scan results showed that at weeks 3, 6 and 12 after surgery, the radioactive counting in radionuclide bone imaging of tubular β-tricalcium phosphate ceramics was higher than that of columnar β-tricalcium phosphate ceramics (P < 0.05). Besides, the radioactive counting in radionuclide bone imaging was increased with time. The result of scanning electron microscopy indicated that at week 3, the shapes of materials kept well; furthermore, host fiber tissue and vein grew from the periphery of materials to inside; at week 6 after surgery, vascular proliferation was active in tubular β-tricalcium phosphate ceramics and widely distributed. Vascular proliferation in columnar β-tricalcium phosphate ceramics was mainly centralized in periphery. At week 12 after surgery, the degree of vascularization in the columnar and tubular β-tricalcium phosphate ceramics was higher, and mature fiber tissues and abundant vascular net were seen in the periphery and center of tubular β-tricalcium phosphate ceramics. While fiber tissues and blood vessel in columnar β-tricalcium phosphate ceramics were mainly centralized in the periphery, and centre shaft were less. These findings suggest that tubular β-tricalcium phosphate ceramics was more helpful to vascularization in vivo than columnar β-tricalcium phosphate ceramics.