黄芪注射液腹腔注射脑缺血再灌注模型大鼠海马组织神经元凋亡及半胱氨酸天冬氨酸蛋白酶3的表达*

背景：有研究表明黄芪注射液可抑制脑缺血再灌注大鼠海马神经元凋亡。 目的：观察黄芪注射液腹腔注射脑缺血再灌注大鼠海马神经元凋亡及半胱氨酸天冬氨酸蛋白酶3的表达。 方法：采用四血管阻断法制备全脑缺血再灌注大鼠模型,建模后给予质量浓度2,4,6,8和10 mL/kg的黄芪注射液腹腔注射,并设立假手术组。分别采用原位末端标记法染色和蛋白免疫印迹方法检测各组大鼠海马神经元凋亡及半胱氨酸天冬氨酸蛋白酶3蛋白表达。 结果与结论：模型组大鼠海马神经元凋亡指数及半胱氨酸天冬氨酸蛋白酶3蛋白表达明显增多(P < 0.05);与模型组相比,黄芪注射液4,6,8,10 mL/kg组海马神经元凋亡指数及半胱氨酸天冬氨酸蛋白酶3蛋白表达明显减少(P < 0.05);与黄芪注射液4 mL/kg组比,黄芪注射液6,8,10 mL/kg 组神经元凋亡指数及半胱氨酸天冬氨酸蛋白酶3蛋白表达减少(P < 0.05),且呈剂量依赖性。结果证实,黄芪注射液可抑制脑缺血再灌注大鼠海马神经元凋亡及半胱氨酸天冬氨酸蛋白酶3蛋白表达。关键词：黄芪注射液;脑缺血再灌注;不同剂量;半胱氨酸天冬氨酸蛋白酶3;细胞凋亡;中医药;组织构建 doi:10.3969/j.issn.1673-8225.2012.20.031

Effect of different doses of astragalus injection on neuronal apoptosis and caspase-3 expression in the hippocampus of rats after cerebral ischemia reperfusion

BACKGROUND: Studies have reported that astragalus injection can inhibit neuronal apoptosis in the hippocampus of rats after cerebral ischemia reperfusion. OBJECTIVE: To investigate the effect of different doses of astragalus injection on neuronal apoptosis and expression of caspase-3 in hippocampus of rats after cerebral ischemia reperfusion. METHODS: Eighty-four SD rats were randomly divided into three groups: the sham operation group, model group and astragalus injection group. The astragalus injection group was then subdivided into five subsets, according to the astragalus injection doses of 2, 4, 6, 8 and 10 mL/kg. The four-vessel occlusion method was used to build a rat model of cerebral ischemia reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining was used to measure neuronal apoptosis in the hippocampus and Western blot was used to measure the expression of caspase-3 protein. RESULTS AND CONCLUSION: Compared with the sham operation group, the apoptosis index and expression of caspase-3 protein increased in the model group (P < 0.05). Compared with the model group, the apoptosis index and expression of caspase-3 protein decreased in the astragalus 4, 6, 8 and 10 mL/kg groups (P < 0.05), but there was no difference in the astragalus 2 mL/kg group (P > 0.05). Compared with the 4 mL/kg group, the apoptosis index and expression of caspase-3 protein decreased in the astragalus 6, 8 and 10 mL/kg groups (P < 0.05) in a dose-dependent manner, but there was no difference among the later three groups (P > 0.05). Astragalus injection can decrease neuronal apoptosis and expression of caspase-3 protein in rat hippocampus after cerebral ischemia reperfusion.