植入式皮质电刺激缺血性脑卒中大鼠皮质神经丝蛋白200的表达

背景：前期研究中发现单独皮质电刺激治疗16 d能改善脑卒中后的运动功能障碍,增加梗死灶周围皮质微管相关蛋白2的表达。目的：观察植入式皮质电刺激对缺血性脑卒中模型大鼠皮质神经丝蛋白200表达的影响。方法：建立SD大鼠右侧大脑中动脉缺血性脑卒中模型,随机分成电刺激组和非刺激组,两组造模1周后植入电刺激器,电极放置在脑梗死区边缘皮质表面,电刺激组施加电刺激,非刺激组不施加电刺激。植入14 d后终止电刺激,6周后用免疫荧光染色和免疫荧光图像分析系统定量分析梗死灶周边、梗死对侧皮质神经丝蛋白200的表达水平。结果与结论：与非刺激组比较,电刺激组梗死灶周边、梗死对侧皮质神经丝蛋白200表达水平均较高(P < 0.05)。表明皮质电刺激可增加梗死灶周边及梗死对侧皮质神经丝蛋白200的表达,诱导双侧皮质神经元轴突的可塑性改变。

Effect of cortical electrical stimulation on neurofilament protein 200 expression in ischemic stroke rats

BACKGROUND:Early studies have shown that cortical electrical stimulation (CES) alone for 16 days can enhance dyskinesia and the expression of microtubule-associated protein 2 (MAP2) in peri-infarct cortex after cerebral ischemia in rats.OBJECTIVE:To investigate the effects of CES on the expression of neurofilament protein 200 (NF-200) in rats model of ischemic stroke.METHODS:Sprague-Dawley rats model of right middle cerebral artery occlusion in ischemic stroke were established. The rats with lesions in the cortex were randomly divided into CES group (n=13) and non-stimulation group (n=10). One week after modeled, electrical stimulators were implanted and the electrodes were placed on the surface of peri-infarct cortex. CES group was given the electric stimulation, but not in the non-stimulation group. CES was terminated after implantation for 14 days. And then the expression of NF-200 in the peri-infarct and contralateral cortex was assessed by immunofluorescence image analysis at 6 weeks after CES started.RESULTS AND CONCLUSION:In the peri-infarct and contralateral cortex, the expression of NF-200 in the CES group was higher than that in the non-stimulation group (P < 0.05). These results suggest that CES can increase the expression of NF-200 in both peri-infarct and contralateral cortex, and induce the plasticity of bilateral cortical neuron axons.