尿酸抑制人骨髓间充质干细胞的成脂分化

背景：研究表明一些药物可影响骨髓间充质干细胞的成骨、成脂诱导分化。目的：观察尿酸对人骨髓间充质干细胞成脂分化的影响。方法：全骨髓培养法体外分离培养人骨髓间充质干细胞,在成脂诱导条件下,分别加入0(对照组),0.1,0.2,0.4,0.8 mmol/L浓度尿酸,诱导14,21 d时行油红O染色,倒置显微镜下计数成脂细胞数量。结果与结论：成脂诱导14 d后,含不同浓度尿酸的诱导组成脂细胞数量较对照组明显减少(P < 0.05),且随着尿酸浓度的增加,抑制成脂细胞生成的效果更为明显(P < 0.05);成脂诱导21 d后,尿酸对骨髓间充质干细胞成脂诱导分化的抑制作用较诱导14 d时更加明显(P < 0.05),同样随着尿酸浓度的增加,抑制作用更加明显(P < 0.05)。说明在体外尿酸能够抑制人骨髓间充质干细胞的成脂分化,并且存在一定的浓度依赖性和时间依赖性。

Uric acid inhibits adipogenic differentiation of human bone marrow mesenchymal stem cells

BACKGROUND:Studies have demonstrated that some drugs can influence the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).OBJECTIVE:To investigate the effect of uric acid on adipogenic differentiation of human BMSCs (hBMSCs).METHODS:BMSCs were isolated and cultured using the whole bone marrow culture method. Under the adipogenic differentiation condition, BMSCs were exposed to five experimental concentrations of uric acid (0 mmol/L(control group), 0.1 mmol/L, 0.2 mmol/L, 0.4 mmol/L, 0.8mmol/L) for 14 days and 21 days. BMSCs were quantitated under phase-contrast inverted microscope after oil red O staining.RESULTS AND CONCLUSION:After 14-days treatment with uric acid in adipogenic medium, BMSCs with positive oil red O staining significantly decreased compared to the control group (P < 0.05) and the inhibitory effect was more apparent as the increase in concentration of uric acid (P < 0.05). The inhibitory effect of uric acid on adipogenic differentiation of BMSCs was more significantly at 21 days than at 14 days (P < 0.05). The inhibitory effect also increased more significantly with increasing concentration of uric acid (P < 0.05). The result reveals that uric acid inhibits the adipogenic differentiation of BMSCs in a dose-dependent and time-dependent manner.