血小板衍化生长因子BB对体外培养肌腱细胞增殖的影响

背景：细胞增殖和组织修复是一个复杂的过程,这期间有大量生长因子参与,并且这些因子在发挥本身功效时,还相互影响和制约,从而更好的促进细胞增殖和组织修复。目的：了解不同质量浓度的血小板衍化生长因子BB对体外培养肌腱细胞增殖的影响。方法：体外培养并鉴定大鼠肌腱细胞,根据培养液中血小板衍化生长因子BB质量浓度的不同分为对照组(0 μg/L)和实验组(1,5,10,20,50,100,150,200,250 μg/L),以MTT法检测细胞增殖能力。另外检测对照组与20 μg/L血小板衍化生长因子BB组培养12-72 h时肌腱细胞的吸光度值,以观察其增殖量效与时间的关系。结果与结论：分离培养的细胞Ⅰ型胶原染色呈阳性, Ⅲ型胶原染色呈阴性,证明为大鼠肌腱细胞。与对照组相比,除 250 μg/L 血小板衍化生长因子BB组外,其他各实验组细胞的吸光度值均升高(P < 0.05或P < 0.01);并且随血小板衍化生长因子BB质量浓度的增加,其吸光度值先逐渐增大,达一定浓度(20 μg/L)后,又逐渐减小。在20 μg/L血小板衍化生长因子BB组,肌腱细胞数量从培养12 h开始增加,至48 h达高峰,在培养24-72 h的吸光度值均显著高于对照组(P < 0.01)。结果可见血小板衍化生长因子BB在一定质量浓度和时间范围内具有促进肌腱细胞增殖的作用。

Effect of platelet-derived growth factor-BB on proliferation of tendon cells cultured in vitro

BACKGROUND:Cell proliferation and tissue repair is a complicated process, accompanied by participation of multiple growth factors. These factors interact with each other to better promote cell proliferation and tissue repair.OBJECTIVE:To investigate the effect of platelet-derived growth factor-BB (PDGF-BB) of different concentrations on proliferation of tendon cells cultured in vitro.METHODS:Rat tendon cells were cultured and identified in vitro. According to concentration of PDGF-BB nutrient solution in culture medium, the cells were divided into control group (0 μg/L) and PDGF-BB groups (1, 5, 10, 20, 50, 100, 150, 200, 250 μg/L). Proliferation of tendon cells was detected by MTT test. The absorbance value of tendon cells in control group and 20 μg/L PDGF-BB group after culture for 12, 24, 36, 48, 60 and 72 hours were determined to evaluate relationship with proliferation dose-effect and time.RESULTS AND CONCLUSION:The isolated cells were identified to be tendon cells as they were positive for type Ⅰ collagen staining and negative for type Ⅲ collagen staining. Compared with control group, the absorbance values of all groups were all increased, except for 250 μg/L PDGF-BB group (P < 0.05 or P < 0.01). Besides, the absorbance value rose gradually with the increase of the concentration of PDGF-BB, and then diminished gradually with the increase of the concentration of PDGF-BB from 20 μg/L. The number of tendon cells in 20 μg/L PDGF-BB group began to increase when cultured for 12 hours, and reached the highest level at 48 hours, and the absorbance at 24-72 hours was significantly greater than control group (P < 0.01). Results suggest that PDGF-BB can promote the proliferation of tendon cells in a definite range of concentration and time．