人脐静脉内皮细胞分离鉴定及其膜分子的表达

背景：肿瘤微环境作用于血管内皮细胞,影响其膜表面分子的表达,与肿瘤的生长、转移及免疫逃逸作用相关,但迄今相关机制尚不完全清楚。目的：分析肿瘤微环境下血管内皮细胞的膜表面分子表达量变化。方法：原代分离培养人脐静脉内皮细胞,采用不同肿瘤培养上清,用不同时间进行诱导。记录培养血管内皮细胞形态;利用免疫组织化学方法,鉴别Ⅷ因子相关抗原;荧光实时定量PCR法测定其膜表面分子mRNA表达量。结果与结论：肝癌smmc7721细胞培养上清处理后,内皮细胞抗原提呈会随着时间延长减弱,黏附白细胞能力随着时间延长而变化。胃癌SGC7901细胞培养上清处理后内皮细胞后,抗原提呈能力无明显变化;黏附能力有关分子中,CD31和细胞间黏附分子1表达降低,CD62E表达增高。说明肿瘤微环境可能通过上述黏附分子和抗原提呈分子表达的变化影响血管内皮细胞的相应功能。

Separation and identification of human umbilical vein endothelial cells and expression of their membrane molecules

BACKGROUND:The tumor microenvironment affects expression of vascular endothelial cells. Critical molecular, which plays a part in tumor growth, metastasis and immunologic escape; however, its specific mechanisms remain largely unknown.OBJECTIVE:To study the expression changes in membrane molecules of vascular endothelial cells under the influence of the tumor microenvironment.METHODS:Human umbilical vein endothelial cells were stimulated with cultured supernatants of tumor cells at the indicated concentration for the same time and with the same concentration for the different time in vitro. Factor VIII related antigen was detected by immunocytochemistry methods. The expression of membrane molecular mRNA was detected by real-time quantitative PCR.RESULTS AND CONCLUSION:The supernatant of hepatocellular cancer cell line smmc7721 suppressed the ability of antigen-presenting and adhesion of vascular endothelial cells. This effect was strengthened when time was increased. However, the supernatant of gastric cancer cell line SGC7901 did not have this effect; it influenced the expression of CD31, CD62E and vascular cell adhesion molecule 1. Tumor microenvironment affects the function of vascular endothelial cells by changing the expression of molecules related antigen-presenting and adhesive capacity.