氨基葡萄糖对白细胞介素1β诱导体外培养人骨关节炎软骨细胞蛋白聚糖代谢的影响

背景：蛋白聚糖和胶原纤维的降解是骨关节炎发病的主要生理和病理学基础,氨基葡萄糖不仅可以减轻骨关节炎疼痛症状,同时可以延缓骨关节炎的病理改变。目的：了解氨基葡萄糖对白细胞介素1β诱导骨关节炎软骨细胞蛋白聚糖代谢的影响。方法：取骨关节炎患者的软骨细胞,分阶段酶消化法进行体外原代培养。在培养液中加入白细胞介素1β诱导剂,设立不含药兔血清对照组、白细胞介素1β对照组和加入不同浓度兔氨基葡萄糖含药血清的实验组。结果与结论：各浓度氨基葡萄糖含药血清组体外培养软骨细胞释放入培养液中糖胺聚糖百分比均值明显低于对照组(P < 0.01),随氨基葡萄糖浓度的增加,糖胺聚糖百分比逐渐降低;蛋白聚糖合成标记物3B3含量的均值较对照组明显增高(P < 0.01),与氨基葡萄糖浓度呈正相关;而蛋白聚糖降解标记物5D4含量的均值则正相反;氨基葡萄糖可以增加骨关节炎患者软骨细胞蛋白聚糖mRNA表达,减低基质金属蛋白酶1,3 mRNA表达。表明氨基葡萄糖可以抑制白细胞介素1β对骨关节炎患者软骨细胞蛋白聚糖代谢的促进作用,达到保护软骨,防止骨关节炎的目的。

Effects of glucosamine on chondrocyte proteoglycan metabolism induced by interleukin-1 beta in human osteoarthritis cultured in vitro

BACKGROUND:Proteolysis of proteoglycan and collagen fiber is the prime basis of physiology and pathology for osteoarthritis. Glucosamine cannot only reduce the pain symptoms but also delay the pathological changes of osteoarthritis.OBJECTIVE:To know about the effects of glucosamine on chondrocyte proteoglycan metabolism induced by interleukin -1β in human osteoarthritis cultured in vitro.METHODS:Chondrocytes extracted from the knee of osteoarthritis patients were primarily cultured by staged enzymatic digestion method. Interleukin-1β was put into the culture medium, and three groups were formed: control group with no medicated rabbit-sera, interleukin-1β control group and experimental group (medicated rabbit-sera were put in various dosages of glucosamine).RESULTS AND CONCLUSION:The percentage of glycosaminoglycanin released in culture solution in the experimental group was significantly decreased compared with the control group (P < 0.01), and glycosaminoglycanin percentage decreased gradually with the increase of glucosamine concentrations. Average value of synthesis marker 3B3 of proteoglycan was obviously increased compared with the control group (P < 0.01), and there was a positive relation with glucosamine concentrations. But the average value of degradation marker 5D4 was inverse. Glucosamine could increase the expression of chondrocyte proteoglycan mRNA and decrease the expression of matrix metalloproteinase-1/matrix metalloproteinase-3 mRNA in the osteoarthritis patients. It shows that glucosamine can inhibit promoting effect of Interleukin-1β on chondrocyte proteoglycan metabolism in osteoarthritis patients to protect the cartilage and prevent osteoarthritis.