兔椎间盘退变模型建立及骨形态发生蛋白7的表达

背景：骨形态发生蛋白7可以促进椎间盘组织合成细胞外基质。目的：观察椎间盘退变兔模型骨形态发生蛋白7的表达。方法：新西兰大白兔采用髓核抽吸法建立椎间盘退变动物模型，对照组暴露相应椎间隙后不进行任何干预。建模成功后，连续核磁共振观察椎间盘变化，12周后生化分析椎间盘中蛋白多糖含量，同时免疫组织化学检测退变的椎间盘组织中Ⅱ型胶原和骨形态发生蛋白7的表达。结果与结论：建模后2周，核磁共振发现模型兔椎间盘开始退变，出现椎间隙狭窄，T2像上椎间盘信号强度减弱。建模后12周，模型兔椎间隙高度、T2信号强度、蛋白多糖含量、Ⅱ型胶原和骨形态发生蛋白7的表达均低于对照组(P < 0.05)。结果证实，实验采用髓核抽吸法成功建立了退变椎间盘兔模型，其退变的椎间盘组织中骨形态发生蛋白7的表达下降。

Construction of an animal model of intervertebral disc degeneration and expression of bone morphogenetic protein-7

BACKGROUND:Bone morphogenetic protein-7 can promote extracellular matrix synthesis in the intervertebral disc tissue.OBJECTIVE:To observe the expression of bone morphogenetic protein-7 in rabbits with intervertebral disc degeneration.METHODS:Models of intervertebral disc degeneration were established in New Zealand white rabbits using nucleus aspiration. The lumbar intervertebral disc of L3/4, L4/5, L5/6 were exposed in control group without treatment. The changes in the intervertebral disc were observed by MRI after modeling. The contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen were measured in 12 weeks later.RESULTS AND CONCLUSION:Intervertebral disc degeneration occurred at 2 weeks after modeling detected by using MRI. Compared with the control group, the disc height, T2 value, contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen in the model group were significantly decreased at 12 weeks after modeling. It indicated that the expression of bone morphogenetic protein-7 was declined in the degenerative intervertebral disc of rabbits induced by nucleus aspiration method.