人胚胎干细胞HuES17向造血干细胞的分化

背景：人类胚胎干细胞是来源于着床前囊胚的内细胞团，能在长期培养中无限增殖并保持未分化状态，且具有分化成人体组织各种细胞类型能力的细胞。 目的：进一步验证人胚胎干细胞HuES17细胞株向造血干细胞分化的能力。 方法：人胚胎干细胞HuES17采用与人包皮成纤维细胞二维共培养的方式培养，采用人胚胎干细胞与小鼠骨髓基质细胞(OP9) 二维共培养的方法诱导胚胎干细胞向造血干细胞分化。 结果与结论：人胚胎干细胞与小鼠骨髓基质细胞(OP9) 二维共培养诱导造血分化的第四五天即开始出现OP9细胞逐渐老化，很快死亡；可以观察到人胚胎干细胞分化，然而，随着OP9细胞死亡，分化的人胚胎干细胞亦死亡，不能诱导人胚胎干细胞向造血干细胞分化。提示人胚胎干细胞HuES17细胞株可能不能向造血干细胞分化，或向造血干细胞分化的能力较低。

Differentiation of human embryonic stem cells HuES17 into hematopoietic stem cells

BACKGROUND:Human embryonic stem cells are derived from inner cell mass of the human blastocyst. The human embryonic stem cells are capable of the unlimited proliferation and maintain the undifferentiated state in long-term culturing in vitro, and can be differentiated into all kinds of cell types of the human body organizations. OBJECTIVE:To study the hematopoietic differentiation of human embryonic stem cells HuES17 cell line. METHODS: Human embryonic stem cells HuES17 were co-cultured with human foreskin fibroblast cells, and the human embryonic stem cells were induced to differentiate into hematopoietic stem cells by co-culturing with bone marrow stromal cells in mice (OP9 cells). RESULTS AND CONCLUSION:When human embryonic stem cells co-cultured with OP9 cells for 5-6 days, the OP9 cells began to aging, and soon died; the differentiation of human embryonic stem cells could be observed. However, with the OP9 cells death, the differentiated human embryonic stem cells were also dead, and could not induce the human embryonic stem cells to differentiate into hematopoietic stem cells. Human embryonic stem cells HuES17 cells line showed little inclination to differentiate into hematopoietic stem cells, or may not be induced to differentiate into hematopoietic stem cells.