人骨髓间充质干细胞及条件培养液对异体T淋巴细胞分泌功能的影响

背景：骨髓间充质干细胞具有支持造血、多向分化及免疫调节作用，后者在异基因造血干细胞移植尤其是HLA半相合造血干细胞移植后移植物抗宿主病中具有潜在的应用价值，但其免疫调节作用的机制不清。 目的：体外观察人骨髓间充质干细胞及其条件培养液对异体外周血T淋巴细胞及其分泌功能的影响。 方法：从人骨髓中分离培养骨髓间充质干细胞，反复贴壁进行纯化；用尼龙棉柱法纯化异体外周血T淋巴细胞。观察骨髓间充质干细胞及其条件培养液体对植物血凝素刺激下T淋巴细胞增殖的影响，以ELISA方法检测γ-干扰素、白细胞介素4水平变化。 结果与结论：骨髓间充质干细胞及其条件培养液体对植物血凝素诱导的T淋巴细胞增殖均有抑制作用，并呈剂量和浓度依赖性。ELISA结果表明，骨髓间充质干细胞及其条件培养液抑制T淋巴细胞γ-干扰素的分泌；骨髓间充质干细胞可促进白细胞介素4的分泌，但其条件培养液对白细胞介素4的分泌无影响。提示骨髓间充质干细胞及其条件培养液对异体外周血T淋巴细胞的增殖有抑制作用，其作用可能与其影响T淋巴细胞分泌γ-干扰素和白细胞介素4有关。

Effects of human bone marrow mesenchymal stem cells and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes

BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) are characterized by supporting hematopoiesis, pluripotential differentiation and immunoregulation. The immunoregulatory effects are of potential importance in prevention and treatment of graft-versus-host disease (GVHD) after transplantation of allogenous hematopoietic stem cells, but the mechanism of immuno-regulation remains unknown. OBJECTIVE:To investigate the immunoregulatory effects of human BMSCs and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes in vitro. METHODS:BMSCs were isolated from human bone marrow and purified by repeated adherence. Allogenous peripheral blood T lymphocytes were harvested using nylon column. The effects of BMSCs and their conditioned medium on the proliferation of phytohemagglutinin-primed T lymphocytes were observed. Interferon-γ and interleukin 4 levels were detected by ELISA. RESULTS AND CONCLUSION:BMSCs and their conditioned medium inhibited the proliferation of phytohemagglutinin-primed T lymphocytes in a dose- and concentration-dependent manner. ELISA results showed that BMSCs and their conditioned medium inhibited the secretion of interferon-γ by T lymphocytes. BMSCs could promote the secretion of interleukin-4, but their conditioned medium did not influence the secretion of interleukin-4. These findings suggest that BMSCs and their conditioned medium inhibit the proliferation of allogenous peripheral blood T lymphocytes, and the inhibitory effects may be related to a fact that BMSCs and their conditioned medium influence T lymphocytes to secrete interferon-γ and interleukin-4.