缩宫素对大鼠产后出血肾脏缺血再灌注损伤病理改变和抗氧化机制的影响

目的探讨缩宫素对妊娠大鼠产后出血致肾脏缺血再灌注损伤的保护作用及其可能的机制。 方法将妊娠足月Wistar大鼠随机(随机数字表法)分为3组，每组30只。即缺血再灌注组、缩宫素处理组和对照组。建立大鼠产后失血性休克再灌注模型，观察肾脏的病理组织学改变并检测血中肌酐(Cr)、血尿素氮(blood urea nitrogen，BUN)的水平和肾组织中丙二醛(MDA)、过氧化物歧化酶(SOD)及谷胱甘肽－过氧化物酶(GSH-PX)3种抗氧化损伤指标的活性。各组间指标的比较采用SNK法分析。 结果(1)缺血再灌注组肾脏间质灶状出血，肾小球萎缩，肾小管肿胀；缩宫素处理组肾脏间质出血明显减轻，肾小球及肾小管结构基本正常。(2)缺血再灌注组Cr(151.58±14.31)μmol/L，BUN(48.73±2.48)mmol/L；缩宫素处理组Cr(83.43±4.67)μmol/L，BUN(37.57±0.50)mmol/L；两组比较差异有统计学意义(q＝3.1551，3.1526；P<0.05)。(3)缺血再灌注组，肾组织中MDA(0.88±0.13)nmol/mg，SOD(28.54±1.31)U/mg，GSH-PX(2.94±0.24)U/mg；而缩宫素处理组MDA(0.12±0.56)nmol/mg，SOD(52.23±2.29) U/mg，GSH-PX(5.83±0.14)U/mg，两组比较差异有统计学意义(q＝3.8293，3.1232，2.9756；P<0.05)。 结论缩宫素对产后失血性休克大鼠再灌注后的肾脏病理改变和肾功能具有一定的保护作用，其机制可能与抑制脂质过氧化反应有关。

The effect of oxytocin on renal ischemia/reperfusion injury induced the pathologic change and antioxidation mechanisms in postpartum hemorrhage rats

ObjectiveTo evaluate the protective role of oxytocin(OT) on protecting the kidney against ischemia/reperfusion injury and its mechanisms in postpartum hemorrhage rats. MethodsWistar rats were randomly divided into 3 groups, which were ischemia/reperfusion group, oxytocin treatment group and control group, respectively. Each group included 30 rats. Postpartum hemorrhage shock rat model was established, and histological changes of kidney samples were observed. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione-peroxidase (GSH-PX) of kidney samples were measured. Creatinine (Cr), blood urea nitrogen (BUN) of blood samples were assayed. The data were analyzed using SNK method. Results(1) In ischemia/reperfusion group, kidneys were local hemorrhage in renal interstitial substance, glomerular atrophy and renal tubule edema. In oxytocin treatment group, local hemorrhage were relieved significantly, and the structure of glomerulus and renal tubule were normal. (2) In ischemia/reperfusion group and oxytocin treatment group, Cr were 151.58±14.31 μmol/L and 83.43±4.67 μmol/L, respectively; and BUN were respectively 48.73±2.48 mmol/L and 37.57±0.50 mmol/L. Compared with two groups, q=3.1551 and 3.1526, p value were all less than 0.05. (3) Compared with ischemia/reperfusion group and oxytocin treatment group, MDA were 0.88±0.13 nmol/mg and 0.12±0.56 nmol/mg; SOD were 28.54±1.31 U/mg and 52.23±2.29 U/mg; and GSH-PX were 2.94±0.24 U/mg and 5.83±0.14 U/mg, respectively. Compared with two groups, q=3.8293, 3.1232 and 2.9756; p value were all less than 0.05. ConclusionsFor renal pathological changes and renal function, oxytocin has protective effect on renal ischemia-reperfusion injury in postpartum hemorrhage shock rats. Inhibiting peroxidation may be their mechanisms.