新型仿生人工神经导管诱导犬周围神经再生过程的组织学观察**

背景：前期动物实验已经证明,精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/β-磷酸三钙/神经生长因子导管可以修复大鼠坐骨神经10 mm缺损,该材料具有良好的生物相容性和细胞亲和性。 目的：观察精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子导管诱导犬周围神经再生过程的组织学变化。 方法：取健康成年杂种犬12只,建立30 mm腓总神经缺损模型,用精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子人工神经导管进行桥接修复。 结果与结论：修复后3个月时在神经远端即可发现新生神经纤维,随着时间的推移,再生神经轴突直径及髓鞘厚度不断增加,9个月时再生神经中可见较多发育成熟的有髓神经纤维。胫骨前肌损伤后出现逐渐萎缩,修复后6,9个月萎缩肌肉逐渐恢复。从组织学角度证实了精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子导管可修复大动物粗大神经缺损。 

Histological observation of dog peripheral nerve regeneration induced by a novel biomimetic nerve conduit

BACKGROUND: Previous research has shown that the novel arginine-glycine-aspatic acid (RGD) peptide modification of poly{(lactic acid)-co-(glycolic acid)-alt-(L-lysine)]}/poly(d,l-lactic acid)/β-tricalcium phosphate/nerve growth factor (PRGD/PDLLA/β-TCP/NGF) composite nerve conduit can effectively repair 10-mm sciatic nerve defects of rats, while it possess good cell affinity and biocompatibility. OBJECTIVE: To observe the histological observation of dog peripheral nerve regeneration with PRGD/PDLLA/β-TCP/NGF composite nerve conduit. METHODS: Twelve healthy hybrid dogs were used as animal models. The conduits were employed to bridge the 30 mm defects in the peroneal nerve. RESULTS AND CONCLUSION: Newborn regenerated myelinated nerve fibers were found in the distal section at 3 months. The diameters of myelinated nerve fibers and thickness of myelin sheath were increased day by day. Tibialis anterior muscles showed gradual atrophy after operation, which peaked 3 months after denervation. Subsequently, the atrophy of tibialis anterior muscles recovered slowly at 6 and 9 months. This study proves the feasibility of the PRGD/PDLLA/β-TCP/NGF nerve conduit for repairing large-diameter nerve defects in large animals.