黄芪多糖对肢体缺血再灌注骨骼肌的保护作用

背景：前期研究表明,黄芪对缺血再灌注心肌细胞的凋亡和损伤皆有影响,其对于骨骼肌缺血再灌注是否有同样作用,国内外尚未见报道。目的：验证黄芪多糖对肢体缺血再灌注骨骼肌的保护作用。方法：取成年家兔按随机数字表法分为2组,建立兔肢体缺血再灌注损伤模型。在即将恢复血流灌注时,对照组及实验组分别自耳缘静脉注射生理盐水、黄芪多糖注射液。分别在缺血前、缺血后2 h、再灌注后1,3 h采集术侧股静脉血样。测定血清乳酸脱氢酶、肌酸激酶活性。取上述各时相点兔胫前肌组织,测定骨骼肌组织湿/干质量比值并观察微细和超微结构变化。结果与结论：实验组再灌注后血清乳酸脱氢酶、肌酸激酶活性明显低于同期对照组(P < 0.01),骨骼肌组织湿/干质量比值低于同期对照组(P < 0.05)。光镜及电镜观察结果示,实验组骨骼肌损伤程度轻于对照组。提示黄芪多糖能够缓解组织水肿,保护缺血再灌注骨骼肌。

Protective effect of Astragalus membranceus polysaccharide on skeletal muscle ischemia-reperfusion injury

BACKGROUND: Previous studies have demonstrated that Astragalus membranaceus can influence the apoptosis and ischemia-reperfusion injury of myocardial cells. However, it is unclear whether Astragalus membranaceus plays the same role in skeletal muscle ischemia-reperfusion injury.OBJECTIVE: To study the protective effect of Astragalus membranceus polysaccharide on skeletal muscle ischemia-reperfusion injury.METHODS: Adult rabbits were randomly divided into experimental and control groups, and a model of limb ischemia-reperfusion injury in rabbits was established. Normal saline injection and Astragalus membranceus polysaccharide were infused intravenously into the rabbits of control and experimental groups, respectively, when blood perfusion was about to be recovered. At preischemia, 2 hours post-ischemia, 1, 3 hours post-reperfusion, blood samples were collected from the operated femoral veins to measure the activities of lactic dehydrogenase and creatine kinase. Meanwhile, the tibialis anterior tissues at the above time points were selected to measure the wet/dry weight ratio of skeletal muscle tissues, as well as to observe fine structure and ultrastructure changes. RESULTS AND CONCLUSION: After reperfusion, the activities of lactic dehydrogenase and creatine kinase in the experimental group were significantly higher than those in the control group at the same time point (P < 0.01). The wet/dry weight ratio of skeletal muscle tissues in the experimental group was lower than that in the control group at the same time point (P < 0.05). The results of an electron microscope and a light microscope showed that the degree of skeletal muscle injury in the experimental group was slighter than that in the control group. These findings suggest that Astragalus membranceus polysaccharide can relieve tissue swelling and protect skeletal muscle from ischemia-reperfusion injury.