用于组织工程骨的磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2微球的合成及检测*

背景：组织工程骨支架常因支架材料的孔隙率不佳和无法以合适的方式提供生物活性因子给种子细胞,导致支架对细胞的黏附及诱导性不强,成骨效率低下。 目的：合成一种新型的可用于组织工程骨的磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2缓释微球并对其各项性能进行检测分析。 方法：复乳法合成磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2微球。 结果与结论：扫描电镜下可见微球呈正圆形,粒径10~100 μm;激光共聚焦显微镜下可见重组人骨形态发生蛋白2在微球上分布均匀;振动样品磁强计检测结果显示微球具有超顺磁性;微球载药量为(1.00±0.18) μg/g;微球对其具有缓释效果;经45 ℃处理微球后测得微球载药量为(0.98±0.20) μg/g。说明合成的磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2缓释微球具有一系列良好的特性,为新型磁性组织工程骨支架的研制奠定了基础。 

Synthesis and detection of magnetic poly lactic acid-glycolic acid copolymer-bone morphogenetic protein 2 microspheres applied in tissue engineered bone

BACKGROUND: Poor porosity and the lack of the ability to provide bioactive factors for seed cells in an appropriate way of tissue-engineered bone scaffolds may lead to weak adhesion and induction of the scaffolds to cells, and low osteogenic efficiency. OBJECTIVE: To synthesis a new kind of magnetic poly lactic acid-glycolic acid copolymer-bone morphogenetic protein 2 (PLGA-BMP2) microspheres that can be used in tissue engineered bone scaffolds and detect their various performance. METHODS: The PLGA-BMP2 microspheres were prepared with double emulsion method. RESULTS AND CONCLUSION: The scanning electron microscopy images showed that the microspheres were positive round with the particle size of 10-100 µm. The laser scanning confocal microscope images showed that human BMP2 evenly distributed on the microspheres. The microspheres had superparamagnetism detected by vibrating sample magnetometer. The microspheres had the sustained-release effect and their drug loading was (1.00±0.18) μg/g. The drug loading was (0.98±0.20) μg/g after the microspheres were treated with 45 ℃. The magnetic PLGA-BMP2 microspheres have a range of good features and lay a foundation for development of newly magnetic tissue engineered bone.