白藜芦醇调控体外培养人骨关节炎滑膜细胞白细胞介素18的表达

背景：白藜芦醇可在体外抑制软骨细胞的凋亡。 目的：观察白藜芦醇对体外培养人骨关节炎滑膜细胞中白细胞介素18、白细胞介素1β和肿瘤坏死因子α表达的影响。 方法：对兔以白藜芦醇临床等效剂量灌胃后,制备含10%,20%,40%白藜芦醇含药血清,与人骨关节炎滑膜细胞共培养,以正常兔血清培养细胞为对照。 结果与结论：ELISA检测及免疫细胞化学检测结果显示,不同浓度白藜芦醇含药血清组体外培养滑膜细胞白细胞介素18、白细胞介素1β、肿瘤坏死因子α分泌量较正常兔血清组明显降低(P < 0.01),并随白藜芦醇浓度的增加,白细胞介素18、白细胞介素1β、肿瘤坏死因子α分泌量逐渐降低(P < 0.01或P < 0.05)。白细胞介素1β、肿瘤坏死因子α水平依次与白细胞介素18水平呈正相关。表明白藜芦醇能够显著下调白细胞介素18、白细胞介素1β、肿瘤坏死因子α在骨关节炎滑膜细胞中的表达,减轻滑膜炎症反应。

Resveratrol down-regulates the expression of interleukin-18 in syoviocytes of osteoarthritis cultured in vitro

BACKGROUND:Resveratrol can inhibit chondrocytes apoptosis in vitro. OBJECTIVE:To observe the effect of Res on the expression of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha in synoviocytes of osteoarthritis cultured in vitro. METHODS:After the rabbits were lavaged with clinical equivalent dose of resveratrol, the 10%, 20% and 40% resveratrol-containing serum were prepared, and then co-cultured with the osteoarthritis synoviocyte. The normal rabbit culture serum was as control. RESULTS AND CONCLUSION:Enzyme linked immunosorbent assay detection and immunocytochemistry results showed that the secretion of interleukin-18, interleukin-1 beta and TNF-α of synoviocyte cultured in vitro in resveratrol-containing serum group was significant lower than that in the non-resveratrol containing serum group (P < 0.01), and with the increasing of resveratrol concentration, the level of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha secretion was gradually reduced (P < 0.01 or P < 0.05). There was a positive correlation between interleukin-18, tumor necrosis factor-alpha and interleukin-1 beta. It indicates that resveratrol can down-regulate the expression of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha in synoviocyte and reduce the inflammatory reaction.