人牙周膜细胞在羧甲基壳聚糖和血小板衍生生长因子BB环境中的增殖

背景：羧甲基壳聚糖或血小板衍生生长因子均可促进对体外培养人牙周膜细胞的增殖。 目的：观察羧甲基壳聚糖和血小板衍生生长因子BB联合应用对体外培养人牙周膜细胞增殖和分化能力的影响。 方法：取生长良好的第4或5代人牙周膜细胞，分组培养：对照组(仅含体积分数2%FBS的DMEM培养液)、10 μg/L 血小板衍生生长因子BB组、100 mg/L羧甲基壳聚糖+10 μg/L 血小板衍生生长因子BB组、800 mg/L 羧甲基壳聚糖+10 μg/L 血小板衍生生长因子BB组、100 mg/L羧甲基壳聚糖组、800 mg/L羧甲基壳聚糖组。 结果与结论：①MTT检测：与对照组比较，其余各组均能促进人牙周膜细胞增殖，且100 mg/L羧甲基壳聚糖+ 10 μg/L血小板衍生生长因子BB、800 mg/L 羧甲基壳聚糖+ 10 μg/L血小板衍生生长因子BB组细胞增殖高于其他组(P < 0.05)，100 mg/L 羧甲基壳聚糖+10 μg/L血小板生性生长因子BB促增殖作用最显著(P < 0.05)。②细胞周期检测：与MTT检测结果相符。③碱性磷酸酶活性：与对照组比较，除10 μg/L 血小板衍生生长因子BB组降低外，其余组均增强(P < 0.05)。表明羧甲基壳聚糖和血小板衍生生长因子BB联合应用可促进人牙周膜细胞增殖和骨向分化。

Effects of carboxymethyl chitosan and platelet-derived growth factor-BB on proliferation of human periodontal ligament cells cultured in vitro

BACKGROUND:Both carboxymethyl chitosan and platelet-derived growth factor-BB can promote the proliferation of human periodontal ligament cells cultured in vitro. OBJECTIVE:To explore the effects of carboxymethyl chitosan and platelet-derived growth factor-BB combination on the proliferation and differentiation of human periodontal ligament cells cultured in vitro. METHODS:The fourth and the fifth generation of human periodontal ligament cells were collected and primarily cultured in different groups: control group (Dulbecco’s modified Eagle’s medium contained 2% fetal bovine serum), 10 μg/L platelet-derived growth factor-BB group, 100 mg/L carboxymethyl chitosan+10 μg/L platelet-derived growth factor-BB group, 800 mg/L carboxymethyl chitosan group+10 μg/L platelet-derived growth factor-BB group, 100 mg/L carboxymethyl chitosan group and 800 mg/L carboxymethyl chitosan group. RESULTS AND CONCLUSION:(1) Compared with control group, each experimental group can promote the proliferation ability of human periodontal ligament cells by MTT assay, and the proliferation of the cells in the combination groups was higher than that in the other groups (P < 0.05), and the dose of 100 mg/L carboxymethyl chitosan plus 10 μg/L platelet-derived growth factor-BB had the greatest promotion effect on the proliferation of human periodontal ligament cells (P < 0.05). (2) The cell cycle results were in line with the results of the MTT assay. (3) Alkaline phosphatase activity detection: compared with the control group, the alkaline phosphatase activity was increased in all groups except for 10 μg/L platelet-derived growth factor-BB group (P < 0.05). It indicates that the combination of carboxymethyl chitosan and platelet-derived growth factor-BB can promote the proliferation and osteogenic differentiation of the human periodontal ligament cells.