基底膜蛋白多糖抗体可抑制体外培养成骨细胞合成及分泌转化生长因子β1

背景：到目前为止,基底膜蛋白多糖在骨折愈合的作用机制、调节转化生长因子β1对成骨细胞增殖的作用尚少见报道。 目的：观察基底膜蛋白多糖对胎鼠颅骨成骨细胞合成分泌转化生长因子β1的影响。 方法：分离并培养SD胎鼠颅盖骨成骨样细胞,采用碱性磷酸酶染色法及茜素红染色法鉴定细胞后,随即分对照组、基底膜蛋白多糖阻断组,应用免疫组化和酶联免疫吸附试验检测基底膜蛋白多糖对成骨细胞分泌转化生长因子β1的情况。 结果与结论：与对照组比较,基底膜蛋白多糖阻断组转化生长因子β1浓度降低,差异有显著性意义(P < 0.05)。说明基底膜蛋白多糖抗体能消除其促进成骨细胞分泌合成转化生长因子β1的作用,使转化生长因子β1浓度降低。

Perlecan antibody can inhibit synthesis and secretion of transforming growth factor beta 1 in osteoblasts cultured in vitro

BACKGROUND:Up to now, there are few reports about the action mechanism of perlecan underlying fracture healing as well as effects of perlecan on proliferation of osteoblasts by regulation of transforming growth factor beta 1 (TGFβ1). OBJECTIVE:To investigate the effect of perlecan on synthesis and secretion of TGF-β1 in osteoblasts from the calvaria of fetal rats cultured in vitro. METHODS:Osteoblast-like cells were isolated from the calvaria of SD fetal rats and cultured. The cultured cells were identified using alkaline phosphatase staining and alizarin red staining. Then, the cells were divided into control and perlecan block groups. The synthesis and secretion of TGF-β1 was examined by using immunohistochemical method and enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION:The level of TGFβ1 was significantly lower in the perlecan block group than the control group (P < 0.05). Perlecan antibody could inhibit the synthesis and secretion of TGFβ1 in osteoblasts cultured in vitro.