壳聚糖-磷酸钙/骨形态发生蛋白2复合骨髓间充质干细胞促进兔脊柱的融合

背景：前期实验已经证明壳聚糖-磷酸钙/骨形态发生蛋白2复合材料能够促进兔脊柱融合。 目的：评价壳聚糖-磷酸钙/骨形态发生蛋白2/碱性成纤维细胞生长因子支架材料在兔椎间融合中的应用效果。 方法：制备壳聚糖-磷酸钙/骨形态发生蛋白2/碱性成纤维细胞生长因子支架材料，并与大鼠骨髓间充质干细胞在体外构成组织工程骨。摘除40只新西兰大白兔椎间盘，随机分为4组：空白对照组未植入任何材料，对照组植入自体髂骨，支架材料组植入壳聚糖-磷酸钙/骨形态发生蛋白2/碱性成纤维细胞生长因子复合材料，实验组植入组织工程骨材料。 结果与结论：术后12周：①X射线片：对照组与实验组椎体融合，两组间融合节段生物力学强度大致相同，生物力学强度高于空白对照组与支架材料组(P < 0.05)，且支架材料组高于空白对照组(P < 0.05)。②组织学切片：实验组与对照组有编织骨岛和新生毛细血管生成，支架材料组仅观察到壳聚糖支架网络，空白对照组未发现特殊组织结构。表明壳聚糖-磷酸钙/骨形态发生蛋白2/碱性成纤维细胞生长因子复合鼠骨髓间充质干细胞能够明显促进脊柱融合。

Chitosan-calcium phosphate/bone morphogenetic protein-2/bone marrow mesenchymal stem cells compound accelerates spinal fusion in rabbits

BACKGROUND:Preliminary experiments have shown that chitosan-calcium phosphate/bone morphogenetic protein-2/basic fibroblast growth factor (CS-CPC/BMP-2/bFGF) scaffolds can promote the rabbit spinal fusion. OBJECTIVE:To evaluate the effect of CS-CPC/BMP-2/bFGF scaffolds on spinal fusion in rabbits. METHODS:The CS-CPC/BMP-2/bFGF scaffolds was produced and co-cultured with bone marrow mesenchymal stem cells to form the tissue engineered bone in vitro. The intervertebral discs of 40 New Zealand white rabbits were removed, and then randomly divided into four groups: the control group was implanted with autogenous iliac bone, the scaffold group was implanted with CS-CPC/rhBMP-2/bFGF composites; the experimental group was implanted with tissue engineered bone-materials; the blank control group was not implanted with any material. RESULTS AND CONCLUSION:After 12 weeks: ①X-ray examination showed the spinal fusion and the biomechanical strength of the fusion segments in the control group and the experimental group were similar, and the biomechanical strength in the control group and the experimental group was stronger than that in the blank control group and scaffolds group (P < 0.05), and the scaffold group was stronger than the blank control group (P < 0.05). ②Histological paraffin sections showed that the woven bone and new blood capillaries could be seen in the experimental group and the control group, and in the scaffold group, we only found the stent mesh of the chitosan; and in the blank control group, we did not find the special structure. The results showed that the CS-CPC/BMP-2/bFGF scaffold combined with bone marrow mesenchymal stem cells could promote the spinal fusion significantly.