骨形态发生蛋白-α-氰基丙烯酸复合生物胶修复大鼠下颌骨骨缺损的研究

背景：骨形态发生蛋白2是骨形态蛋白家族中诱导成骨作用最强,并能单独诱导成骨的因子。 目的：观察骨形态发生蛋白-α-氰基丙烯酸正丁酯复合生物胶修复大鼠下颌骨缺损的效果。 方法：将54只Wistar大鼠随机分为实验组、对照组、空白对照组。3组均制作下颌骨缺损模型,实验组缺损处植入骨形态发生蛋白-α-氰基丙烯酸复合生物胶,对照组植入壳聚糖温敏性凝胶,空白对照组不植入任何材料。 结果与结论：①X射线片检查：植入后3,6,9 周时,实验组剩余骨缺损面积均低于对照组及空白对照组(P < 0.05)。②组织学观察：病理切片显示,实验组下颌骨成骨情况优于对照组及空白对照组。表明骨形态发生蛋白-α-氰基丙烯酸复合生物胶具有良好的生物相容性和骨诱导性,可显著促进颌骨缺损修复。

Compound bone morphogenetic protein-alpha-cyanoacrylate biological glue repairs mandibular defects in rats

BACKGROUND:Bone morphogenetic protein 2 is the strongest in the family of bone morphogenetic proteins which can induce osteogenesis separately. OBJECTIVE:To evaluate the effectiveness and feasibility of implantation of the compound bone morphogenetic protein- cyanoacrylate biological glue in the reconstruction of rat mandibular defects. METHODS:Totally 54 male Wistar rats were divided into three groups randomly and equally: The experimental group, the control group (the chitosan group) and the blank group. Mandibular defects of rats were repairing by implanting the compound bone morphogenetic protein-α-cyanoacrylate biological glue in the experimental group. While mandibular defects of the chitosan group were repaired only with chitosan. The blank group received no implantation. RESULTS AND CONCLUSION:①X-ray examination: At weeks 3, 6, 9 postoperation, the area of residual mandibular defects in the experimental group was smaller than that of the other two groups. There was a statistically significant difference between the experimental and the other two groups (P < 0.05). ②Histopathology observation: The new bone mass in the experimental group was superior to those in the other two groups. It is demonstrated bone morphogenetic protein α-cyanoacrylate biological glue can be used to restoration of rat mandibular bone defects and significantly promote the mandibular repair.