木瓜蛋白酶诱导兔膝关节骨关节炎模型滑膜中白细胞介素1、白细胞介素6、白三烯浓度变化与药物注射时间的关系

背景：木瓜蛋白酶诱导兔膝关节骨性关节炎的造模方法可以获得稳定的退行性关节炎模型。 目的：观察兔膝关节腔内注射木瓜蛋白酶诱导骨关节炎后，滑膜组织中白细胞介素1，6、白三烯的水平变化与药物注射时间的关系。 方法：将18只雄性新西兰大白兔随机数字表法均分为2周组、4周组、6周组。将木瓜蛋白酶水溶液分别在实验开始的第1，4，7天分别注射入兔右膝关节腔内，建立膝关节骨性关节炎模型，于首次药物注射后第2，4，6周3个时间点分别将相应组实验动物处死后检测膝关节滑膜白细胞介素1，6、白三烯浓度。同时切取2周组造模兔的左膝正常滑膜组织作为正常对照组。 结果与结论：2周时白细胞介素1，6、白三烯水平比正常组升高，差异有显著性意义(P < 0.05)；4周时白细胞介素1，6 、白三烯水平与2周组相比较，差异无显著性意义(P > 0.05)。6周时白细胞介素1，6水平与正常组比较，差异无显著性意义(P > 0.05)。6周时白三烯水平比正常组升高(P < 0.05)；比4周时降低(P < 0.05)。说明兔膝关节首次注射木瓜蛋白酶后4周内炎症因子水平变化显著。

Relationship of synovium interleukin-1, interleukin-6 and leukotriene level changes to injection timing in rabbits with papain-induced knee osteoarthritis

BACKGROUND:A stable degenerative knee osteroarthritis model in rabbits can be established by the induction of papain. OBJECTIVE:To ivestigate the relationships of the level changes of synovium interleukin-1 interleukin-6 and leukotriene to injection time in rabbits with knee osteoarthritis induced by intraarticular injection of papain. METHODS:Totally 18 New Zealand male white rabbits were divided into 2 weeks group, 4 weeks group and 6 weeks group randomly (6 rabbits in each group). Papain solution was injected into the right knee joint cavity of each rabbits at days 1, 4 and 7, and rabbit model of knee osteoarthritis was established. The rabbits of corresponding groups were killed at weeks 2, 4 and 6 after first injection. And then, interleukin-1, interleukin-6 and leukotriene concentrations of right knee synovium were examined. Normal rabbit left knee synovium of the 2 weeks group were examined simultaneously as the control group. RESULTS AND CONCLUSION:Level of interleukin-1, interleukin-6 and leukotriene at week 2 was higher than those of the control group. There was significant difference between the former two groups (P < 0.05). In terms of the levels of interleukin-1, interleukin-6 and leukotriene, there was no significant difference between the 4 week and 2 week groups (P > 0.05). There was no significant difference between the 6 week group and the control group in the level of interleukin-1 and interleukin-6 (P > 0.05). The leukotriene of the week 6 group was higher than that of the control group (P < 0.05), and was lower than that of the 4 week group (P < 0.05). These results suggest that the level changes of synovial inflammatory factors in rabbit knee joint are significantly increased within 4 weeks after the first injection of papain.