血小板裂解液在骨组织工程中的应用

 背景：以血小板裂解液修复骨缺损不仅去除了残余细胞结构,降低了免疫原性,而且还保留了其中的多种生长因子,可为异体或异种移植创造条件。 目的：总结归纳有关对能促进骨生长的血小板裂解液相关研究及应用方式进行综述。 方法：以“tissue engineering, bone defect, gene therapy, growth factor”为检索词,检索PubMed数据库(2004-01/2011-01),以“血小板血浆,骨组织工程,血小板裂解液,生长因子”为检索词,检索CBM数据库(2004-01/2011-01)。文献检索语种为英文和中文。纳入血小板裂解液促进骨的再生和修复的文献,排除重复文献。 结果与结论：计算机初检得到340篇文献,根据纳入排除标准,对其中23篇文献进行分析。目前研究证实,血小板裂解液可促进骨髓基质干细胞和成骨细胞的增殖;与生物陶瓷或自体骨复合,可明显促进骨缺损的修复。合理应用血小板裂解液对骨的再生和修复具有重要意义。关键词：血小板裂解液;成骨细胞;骨缺损;细胞生长因子;骨组织工程 doi:10.3969/j.issn.1673-8225.2012.20.041

Platelet lysate’s advances in bone tissue engineering

BACKGROUND: Platelet lysates to repair bone defects cannot only remove the residual cell structure, reduce immunogenicity, but also retain many growth factors to create the conditions for the allograft or xenograft. OBJECTIVE: To review the studies that platelets can promote the growth of bone lysis as well as application methods in relevant research. METHODS: PubMed (2004-01/2011-01) and CBM databases (2004-01/2011-01) were searched by using the keywords of “tissue engineering, bone defect, gene therapy, growth factor” in English and “platelet plasma, bone tissue engineering, platelet lysate, growth factor” in Chinese, respectively. Studies addressing platelet lysate to promote bone regeneration and repair were included, and repetitive studies were excluded. RESULTS AND CONCLUSION: Totally 340 articles were retrieved, and only 23 articles met the inclusive criteria. Up to now, studies have demonstrated that platelet lysate can promote the proliferation of bone marrow mesenchymal stem cells and osteoblasts. Platelet lysate combined with biological ceramic or autogenous bone can significantly promote the repair of bone defects. Rational use of platelet lysate in bone regeneration and repair is of great significance.Chen L, Li QJ, Song HP. Effects of brain microvascular endothelial cells and astrocytes following treatment with baicalin on neural stem cell differentiation.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(20): 3793-3796.