成骨诱导人脐带间充质干细胞与纳米羟基磷灰石/聚酰胺66支架材料的生物相容性☆

背景：纳米羟基磷灰石/聚酰胺66材料有利于成骨细胞的长入和新生骨的形成、且抗弯强度、抗压强度等各项参数与正常骨组织的力学性能相接近，能满足实验动物硬组织修复的要求。 目的：分析成骨诱导后人脐带间充质干细胞与纳米羟基磷灰石/聚酰胺66复合支架的生物相容性。 方法：体外培养人脐带间充质干细胞，纯化增殖，成骨诱导。取成骨诱导后的第3代人脐带间充质干细胞接种于纳米羟基磷灰石/聚酰胺66支架材料上，观察细胞的生长、增殖情况及材料细胞毒性。 结果与结论：成骨诱导后人脐带间充质干细胞在复合支架上生长分化良好，增殖活性不受材料影响。成骨诱导14 d内，可见碱性磷酸酶活性随着培养时间延长而逐渐增高。MTT法检测细胞无毒性。扫描电镜观察，1 d后可见细胞在支架表面附着生长；7 d后可见细胞在材料上生长良好，材料空隙有大量充填。说明纳米羟基磷灰石/聚酰胺66支架可作为骨组织工程中人脐带间充质干细胞的细胞载体，具有良好的生物相容性，能满足骨组织工程的需要。关键词：羟基磷灰石/聚酰胺66；人脐带间充质干细胞；细胞培养；骨组织工程；支架；生物相容性 缩略语注释：nHA/PA66：nano-hydroxyapatite crystals and pnolyamide 66，羟基磷灰石/聚酰胺66；hUCMSCs：human umbilical cord mesenchymal stem cells，人脐带间充质干细胞 doi:10.3969/j.issn.1673-8225.2012.16.019

Biocompatibility of nano-hydroxyapatite/polyamide 66 materials composite with human umbilical cord mesenchymal stem cells after osteogenic induction

BACKGROUND: Nano-hydroxyapatite/polyamide (nHA/PA) 66 materials are beneficial to osteoblast regeneration and new bone formation. Their bending strength and compressive strength are close to mechanical properties of normal bone tissues to meet the needs of hard tissue repair in animal experiment. OBJECTIVE: To explore the biocompatibility of nHA/PA66 materials composite with human umbilical cord mesenchymal stem cells (hUCMSCs) after osteogenic induction. METHODS: The hUCMSCs were isolated, cultured and osteoblastic-induced in vitro. The third-generation hUCMSCs were seeded on the nHA/PA66 materials. The growth and proliferation of hUCMSCs and cytotoxicity materials were observed. RESULTS AND CONCLUSION: The osteoblastic-induced hUCMSCs grew well on the composite scaffolds, and proliferative activity of the cells did not change. The levels of alkaline phosphatase were gradually increased as the time of culture went on in the first 14 days of osteogenic induction. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results showed that the cells were atoxic. Scanning electron microscopic observation: the cells showed the attached growth on the surfaces of nHA/PA66 scaffolds at 1 day; the cells grew well on the surface of nHA/PA66 scaffold and extended into the interspace of the scaffolds at 7 days. The nHA/PA66 materials can be used as cell carrier for hUCMSCs. They can satisfy the demand of bone tissue engineering due to their good biocompatibility.