重组人白细胞介素10抗家兔皮肤移植排斥反应中Toll样受体的变

背景：现已认识到Toll样受体的活化与器官移植后排斥反应的关系十分密切,Toll样受体与移植免疫也逐渐成为研究的热点问题。目的：观察重组人白细胞介素10抗皮肤移植排斥反应中受体兔血清Toll样受体的变化。方法：制备兔背部缺损模型,将受体家兔分为6组,重组人白细胞介素10低、高剂量组、环孢素A低、高剂量组、联合用药组和生理盐水组,分别于移植前1 d开始肌肉注射5,10 μg/(kg•d)重组人白细胞介素10,5,10 mg/(kg•d)环孢素A,5 μg重组人白细胞介素10+5 mg环孢素A,1 mL/d生理盐水,连续用药10 d。结果与结论：各组移植后Toll样受体4,7,8,9水平均较移植前升高,移植后14 d达到高峰。移植后4,7 d生理盐水组Toll样受体4水平高于联合用药组(P < 0.05)。移植后7 d生理盐水组Toll样受体7高于其他各组(P < 0.05),Toll样受体8高于环孢素A高剂量组(P < 0.05)。各组移植不同时间点Toll样受体9水平差异无显著性意义(P > 0.05)。提示重组人白细胞介素10抗家兔皮肤移植反应中,Toll样受体4,7,8,9水平在皮肤移植后明显升高,当皮片排斥后达到高峰,之后逐渐下降,表明Toll样受体参与排斥反应过程。

Changes of Toll like receptors in the reaction of recombinant human interleukin-10 resisting rabbit skin grafting

BACKGROUND:The researchers have recognized that there is a very close relationship between the activation of the Toll-like receptors and organ transplant rejection, and the Toll-like receptors and transplantation immunology have gradually become a hot topic.OBJECTIVE:To observe the changes of Toll-like receptors in the serum drawing from rabbits treated with recombinant human interleukin-10 after allogeneic skin grafting.METHODS:The rabbit back defect models were prepared, and the receptor rabbits were divided into six groups: low dose recombinant human interleukin-10 group, high dose recombinant human interleukin-10 group, low dose cyclosporine A group, high dose cyclosporine A group, joint group and normal saline group. The 5 and 10 μg/(kg•d) recombinant human interleukin-10, 5 and 10 mg/(kg•d) cyclosporine A, 5 μg recombinant human interleukin-10+5 mg cyclosporine A and 1 mL/d normal saline were injected into the rabbits respectively from 1 day before grafting and lasted for 10 days.RESULTS AND CONCLUSION:The levels of serums Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased after skin grafting compared with that before grafting and reached to the peak concentration on 14 days. At 4 and 7 days after grafting, the level of Toll-like receptors 4 in the normal saline group was higher than that in the joint group (P < 0.05). At 7 days after grafting, the level of Toll-like receptor 7 in the normal saline group was higher than that in the other five groups (P < 0.05), and the level of Toll-like receptor 8 in the normal saline group was higher than that in the high dose cyclosporine A group (P < 0.05). There was no significant difference of Toll-like receptor 9 level in each two groups at different time points after grafting (P > 0.05). The levels of serum Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased obviously after the rabbits skin grafting which have treated with recombinant human interleukin-10 resisting skin grafting, and reached to the peak concentration after skin graft rejection, and then decreased gradually, which means Toll-like receptors participate in the allograft rejection.