贵金属烤瓷合金与高糖对细胞外液炎性因子的影响 |
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作者姓名: | 谢 晖 丁江峰 |
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作者单位: | 广州医学院口腔医院,广东省广州市 510140 |
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摘 要: | 背景:前期研究中发现高糖与烤瓷合金浸提液对小鼠成纤维细胞L-929的增殖存在交互效应。
目的:观察3种贵金属烤瓷合金与高糖对小鼠成纤维细胞L-929中白细胞介素6水平的影响。
方法:采用两因素析因设计实验研究不同糖浓度(11.1,22.2,33.3 mmol/L)下,89%金合金、74%金合金及银钯合金贵金属烤瓷合金浸提液,分别与小鼠成纤维细胞L-929体外共培养24h后,细胞外液中白细胞介素6水平的变化,并设未加入合金材料的空白对照为阴性对照组。
结果与结论:两因素析因设计分析结果表明,贵金属烤瓷合金浸提液的主效应有统计学意义(P=0.001),其中74%金合金与银钯合金白细胞介素6水平低于阴性对照组(P=0.004,0);糖浓度的主效应也有统计学意义(P=0.005), 33.3 mmol/L糖浓度组白细胞介素6水平低于11.1 mmol/L(正常)糖浓度组(P=0.001)。但贵金属烤瓷合金浸提液与糖浓度两因素对小鼠成纤维细胞L-929细胞外液中白细胞介素6水平的影响无交互作用(P=0.33)。
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关 键 词: | 贵金属 烤瓷合金 银钯合金 金合金 浸提液 细胞外液炎性因子 高糖浓度 交互作用 小鼠成纤维细胞 |
收稿时间: | 2012-04-30 |
Effects of noble ceramic alloy and high glucose on inflammatory factors in the extracellular fluid |
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Authors: | Xie Hui Ding Jiang-feng |
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Institution: | The Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, Guangdong Province, China |
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Abstract: | BACKGROUND:The preliminary studies have found that there is an interaction effect of high sugar and ceramic alloy extracts on proliferation of L-929 mouse fibroblasts.
OBJECTIVE:To observe the effects of three kinds of noble ceramic alloys and high glucose on secrets of interleukin-6 from L-929 cells.
METHODS:Using two-factor factorial design experiments, we detected the changes of interleukin-6 levels in the extracellular fluid after co-culture of 89% Au-alloy, 74% Au-alloy and Ag-Pd alloy with mouse L-929 cells under different glucose concentrations (11.1, 22.2, 33.3 mmol/L). Cells without alloy served as negative controls.
RESULTS AND CONCLUSION:Two-factor factorial design results showed that the main effect of noble ceramic alloy extracts was statistically significant (P=0.001). The interleukin-6 levels of the supernatant in the 74% Au-alloy and the Ag-Pd alloy were significantly lower than that in the negative group (P=0.004, 0). The main effect of glucose concentration was also of statistical significance. The interleukin-6 level in supernatant in 33.3 mmol/L high glucose was significantly lower than that in 11.1 mmol/L glucose. However, no interaction existed between different extracts of noble ceramic alloys and glucose concentrations about the secrets of interleukin-6 from L-929 cells (P=0.33). |
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