连翘苷对MPP+诱导人神经母细胞株SH-SY5Y细胞损伤的保护作用

目的：研究连翘苷对1-甲基-4-苯基吡啶离子（1-methyl-4-phenylpyridinium，MPP⁺）诱导人神经母细胞株SH-SY5Y细胞损伤的神经保护作用。方法：培养的SH-SY5Y细胞加入不同浓度的连翘苷，以MTT代谢率为指标确定连翘苷的无毒范围；对培养的SH-SY5Y细胞加入连翘苷100，10，1 μmol·L^-1预孵育24 h，加入1 mmol·L^-1 MPP⁺ 作用48 h诱导损伤，观察MTT代谢率、乳酸脱氢酶（Lactate dehydrogenase，LDH）漏出率的改变。结果：连翘苷在1~100 μmol·L^-1浓度范围内不影响SH-SY5Y细胞存活率；与正常对照组细胞相比，MPP⁺损伤模型组MTT代谢率明显降低（P＜0.001），LDH漏出率显著增加（P＜0.001）；与模型组相比，连翘苷各组细胞活性显著升高（P＜0.05），LDH漏出率明显降低（P＜0.01）。结论：连翘苷对MPP⁺诱导的SH-SY5Y细胞损伤具有神经保护作用。

Effects of Phillyrin on MPP+-induced Injury in SH-SY5Y Neuroblastoma Cells

Objective：To investigate the effects of Phillyrin on 1-methyl-4-phenylpyridinium (MPP⁺) -induced injury in SH-SY5Y neuroblastoma cells. Methods：SH-SY5Y cells were incubated with Phillyrin of different concentrations. MTT metabolic rate was measured as the cell survival rate to determine the effective concentrations of Phillyrin. After SH-SY5Y cells were incubated with Phillyrin (1，10，100 μmol·L^-1) for 24 h prior to exposure to MPP⁺ (1 mmol·L^-1) for 48 h，the MTT metabolic rate and LDH leakage rate were observed. Results：Phillyrin did not decrease the survival rate of SH-SY5Y cells at the concentrations from 1 to 100 μmol·L^-1. Compared with the control group，MPP⁺ decreased the MTT metabolic rate （P<0.001） and increased the LDH leakage rate (P<0.001) of SH-SY5Y cells. However，Phillyrin significantly increased the cell activity (P<0.05) and decreased LDH leakage rate of SH-SY5Y cells with MPP⁺-induced injury. Conclusion：Phillyrin protects SH-SY5Y cells against MPP⁺-induced cytotoxicity.