纤维素-大豆蛋白混合膜与内皮细胞生物相容性的实验研究

目的:研究和评价纤维素-大豆蛋白混合膜与内皮细胞的生物相容性,为其作为人工生物支架材料应用于组织工程提供实验依据。方法:将内皮细胞株ECV304与不同组分、碱处理前后的纤维素-大豆蛋白混合膜共培养,分别以四氮甲唑蓝(MTT)法检测细胞活力,以扫描电镜(SEM)观察细胞形态和生长状态。结果:与阴性对照组和单纯纤维素组相比,ECV304在纤维素-大豆蛋白混合膜上生长良好,大豆蛋白加入到纤维素膜中显著提高了ECV304的细胞活力;在碱处理后的混合膜表面,细胞长出许多足突伸入膜孔隙,明显促进细胞在膜表面粘附。结论:初步表明纤维素-大豆蛋白混合膜与内皮细胞ECV304生物相容性良好,在血管组织工程领域具有应用潜能。

The Experimental Study on Biocompatibility of the Cellulose-Soy Protein Blend Membrane and Endothelium

Objective:The effect on the biocompatibility of the soy protein content in blend membrane,alkali-treatment and the endothelium cells(ECV304) were investigated primarily,to ealuate the biocompatibility of the cellulose-soy protein blend membrane for the potential applications in the tissue engineering field.Method:The ECV304 cells were cultured on the surfaces of the cellulose-soy protein blend membrane with or without alkali-treatment.The MTT assay was used to evaluate the cell viability,and the morphology and growth status of the cells which on the surfaces of the membrane were observed by scanning electron microscope(SEM).Results:Comparing with the control group and the pure cellulose membrane group,the incorporation of soy protein into cellulose could enhance the cell viability of ECV304,and the cells extended the pedicels into the pore of the membrane after alkali-treatment to the blend membrane,increased the density of cells adhered on the surface of the membrane.Conclusion:The results revealed that the cellulose-soy protein blend membrane exhibits good biocompatibility to the ECV304 cells,it showed potential application in the field of vascular tissue engineering.