Characterization of peripheral blood TCR repertoire in patients with ankylosing spondylitis by high-throughput sequencing |
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Authors: | Jin-Huan Cui Ya-bin Jin Kai-Rong Lin Ping Xiao Xiang-ping Chen Ying-ming Pan Wei Lin Zu-chang Wu Dong-mei Guo Xiao-fan Mao Chu-ling Zhang Wen-lue Lian Wei Luo |
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Affiliation: | 1. Clinical Research Institute, Foshan Hospital, Sun Yat-sen University, Foshan 528000, Guangdong, China;2. Department of Rheumatology, Foshan Hospital, Sun Yat-sen University, Foshan 528000, Guangdong, China |
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Abstract: | Ankylosing spondylitis (AS) is a chronic and progressive autoimmune disease affecting the invasion of the spine, sacroiliac joints and peripheral joints. T cells play a vital role in the underlying pathogenesis of AS, which mediated autoimmune and inflammatory responses via specific recognition of autoantigen peptides presented by susceptibility HLA. Antigen-specific T cells triggered by HLA/antigen complexes will undergo a massive expansion that forming an uneven T cell repertoire. To enhance our understanding of T-cell-mediated autoimmune in AS, we applied TCR β chains high-throughput sequencing to AS patients for in-depth TCR repertoire analysis. A significantly lower TCR repertoire diversity was observed in peripheral blood of AS patients relative to controls. And severe patients in our AS cohort have a more restricted TCR repertoire than mild patients, suggesting that the TCR repertoire diversity might be associated with the clinical severity of disease. No V, J and VJ pairs with significant biased usage were identified, which indicated that the usage frequency deviation of certain V/J/V-J genes in AS patients is little. This is a pilot study with potentially interesting observation on reduced diversity of T cells repertoire in peripheral blood of AS patients and further studies are needed. |
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Keywords: | Ankylosing spondylitis (AS) Clonotypes Diversity T cell receptor (TCR) TCR repertoire |
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