饮水矿物质水平对大鼠亲3代繁殖及子3代发育的影响研究

目的 比较矿物质水平差别显著的3种饮水对大鼠繁殖能力和子代发育的影响.方法 将45只雄性和90只雌性断乳SD大鼠按随机数字表法分为3组(雌雄比为2∶1),分别饮用A水溶解性总固体(total dissolved solids, TDS)为1.2 mg/L]、B水(TDS:87.2 mg/L)以及经净水器(活性炭滤芯)处理自来水后得到的C水(TDS:291.0 mg/L),连续3代.逐代比较亲代繁殖指标和子代发育指标.结果 亲3代的繁殖指标3组间均未见统计学差异.A水组仔鼠出生后4 d存活率至F3代时明显低于其他2组.而C水组仔鼠出生后4 d存活率不仅在F1代时显著高于B水组(P<0.01),还在F3代时显著高于其他2组,该组在F1代时记忆能力也有高于其他2组的趋势.结论 多代连续饮用矿物质极低的饮水对大鼠繁殖能力影响不明显,但低矿水对子代早期发育水平可能有不利影响.对于大鼠出生后的早期生长阶段,饮用卫生学上安全且富含矿物质的饮水将对其发育更为有利.

Comparison of 3 types of drinking water with different mineral levels on 3 generational reproductive and developmental outcomes in rats

Objective To compare the multi-generational reproductive and developmental outcomes in rats which consume 3 types of drinking water with different mineral levels.Methods Ninety female and forty-five male weaning SD rats were randomly divided into 3 groups, with the ratio of female to male of 2 to 1 per group.The 3 groups were given 3 different waters separately from the weaning rat till to whose F3 generation pups weaning.The animals were allowed free access to food and water.The 3 types of water were water A total dissolved solids (TDS), 1.2 mg/L], water B (TDS 87.2 mg/L) and C (TDS 291.0 mg/L).Parental reproductive outcomes and offspring developmental parameters were observed and compared in each generation.Results There were no statically differences in the parental reproductive parameters in all the 3 groups from F0 to F2 generations.The offspring survival rates (OFSR) on postnatal days (PND) 4 in F3 generation were statistically lower in water A group than the other 2 groups (P<0.01).While the OFSR on PND4 of the water C group was not only obviously higher in F1 generation than those of the water B group (P<0.01), but also notably higher in F3 generation than those of the other 2 groups.What's more, the learning ability of the pups in water C group was higher than the other 2 groups.Conclusion Drinking water which hardly contains minerals might have no effect on the parental reproductive ability, but have negative effect on the early development of the offspring.For the rats in early period of development, drinking water with hygienic safety and rich minerals is beneficial to the development.