980nm连续激光在金属医疗器械表面高效灭菌的实验研究

目的 探讨980 nm红外连续激光对金属医疗器械表面高效灭菌的功率和时间阈值关系.方法 采用人工污染实验法,以无菌钢制手术刀为载体,用金黄色葡萄球菌和大肠杆菌分别进行污染,经不同功率、对应不同辐照时间的激光消毒灭菌后用细菌培养基清洗刀片采样,采用增菌培养来检测红外激光对大肠杆菌和金黄色葡萄球菌的灭活情况.结果 980 nm激光在5.24 W时辐照29 s,6.87 W时辐照24 s,8.33 W时辐照14 s,10.11W时辐照14 s都能彻底杀死金黄色葡萄球菌;980 nm激光在5.24 W时辐照22 s,6.87 W时辐照12s,8.33W时辐照11s,10.11W时辐照9s都能彻底杀死大肠杆菌.大肠杆菌比金黄色葡萄球菌更易灭活.依据实验数据拟合,在同一激光功率下,两种细菌存活率与辐照时间的函数符合一阶反应动力学形式.结论 980 nm红外激光能够实现金属医疗器械表面的快速消毒灭菌,并且功率越高则时间越短、效果越好.

Sterilizing effect of 980 nm continuous laser on metal surface of medical equipment

Objective To investigate the relationship of power and exposure period of 980 nm continuous laser system on the surface sterilization for metal medical equipment.Methods The sterile steel lancets were artificially contaminated with Staphylococcus aureus and Escherichia coli,respectively.These lancets were exposed and disinfected by 980 nm continuous laser at different powers for different time periods.The inactivation of the bacteria on each lancet was observed and measured by 8-hour enrichment culture.Fesults Staphylococcus aureus were thoroughly killed after the lancets were disinfected by the 980 nm laser at 5.24 W for 29 s,6.87 W for 24 s,8.33 W for 14 s,and 10.11 W for 14 s,respectively.The power and exposure period for Escherichia coli were 5.24 W for 22 s,6.87 W for 12 s,8.33 W for 11 s,and 10.11 W for 9 s,respectively.Escherichia coli was more likely to be inactivated than Staphylococcus aureus.According to the experimental data,the survival rate under same power of infrared laser as a function of irradiation time was fitted to the first-order kinetics equation.Conclusion 980 nm infrared laser can achieve rapid sterilization on metal surface of medical equipment in a very short time period.The larger power the laser is with,the shorter is the exposure period,and the better the sterilizing effect is.