Plackett-Burma联用正交设计优选蛹虫草多糖制备工艺

目的 Plackett-burman试验(P-B试验)方法联用正交试验优选蛹虫草多糖最佳提取工艺.方法 在单因素试验的基础上,通过P-B试验筛选出影响蛹虫草多糖提取率的显著因素,通过3因素3水平正交试验,获得蛹虫草多糖的最佳提取工艺.结果 通过P-B试验得出料液比、提取时间及提取温度是影响蛹虫草多糖提取率的显著因素,正交试验结果显示蛹虫草多糖最佳提取工艺为:料液比1∶62.5、提取时间3 h、提取温度90 ℃、提取次数为2次、乙醇浓度95%、沉淀时间24 h,提取率为2.145%.结论 P-B试验通过比较因子水平的差异与整体的差异来确定因子的显著性,减少了试验次数.

Plackett-Burma combined with orthogonal design to optimize the preparation technology of cordyceps militaris

Objective To optimize the experimental conditions of extracting polysaccharides in cordyceps militaris.Methods Taking extraction yield as the index to optimize the production process,and combining the orthogonal design method with Plackett-Burma based on the single factor experiment.Results The significant factors influencing the cordyceps extraction yield was material to liquid ratio,extracting time and extractingtemperature.The optimal production technology as follow:the ratio of cordyceps militaris and water was 1∶62.5,and extracting timewas 3 h,extractingtemperaturewas 90 ℃,extracting times was 2 times,ethanol concentration 95%,settling time for 24 hours,the extraction yield of 2.0767%.Conclusion Plackett-burman test could determine the significance of factorby comparing the difference among the factorslevels and the overall,and reduce the testnumber.