心理应激对动脉粥样硬化大鼠炎症反应的影响及意义

目的：观察心理应激对免疫反应的影响，探讨心理应激在斑块稳定性中的作用、机制及临床意义。方法：将24只雄性SD大白鼠按体重随机分为正常对照组、无应激组、生理应激组和心理应激组，每组6只，并制作动脉粥样硬化和心理应激模型。用酶联免疫吸附法测定血清sICAM-1浓度；用免疫组化法测定主动脉内ICAM-1表达情况。结果：（1）心理应激组大鼠血清sICAM-1浓度较其他各组明显升高（均P〈0．05）；（2）心理应激组大鼠升主动脉壁ICAM-1表达较其他各组明显升高（均P〈0．01）；（3）血清sICAM水平与动脉壁内ICAM-1表达之间存在正相关关系（r=0．747，P〈0．01）。结论：（1）心理应激可直接影响全身和局部的炎症反应；（2）在心理应激者可利用血液中炎症指标判定斑块局部的炎症状态进而推断其稳定性；（3）干预心理应激因素可能会预防动脉炎症的发生发展。

Effect and significance of psychological stress on inflammatory response in atherosclerotic rats

Objective To explore its importance,mechanism,and clinical significance in atherosclerotic plaque instability by studying the effect of psychological stress on inflammatory response in atherosclerotic rats.Methods According to the rats weight,24 healthy male SD rats were randomly divided into four groups,control group,the no-stress group,the physical stress group and the psychological stress group.The atherosclerosis and the stress models were made.The blood samples were tested by enzyme-link-immuno absorption method,and the express of ICAM-1 in ascending aortic artery were tested with immunohistorychemitry.Results The serum level of sICAM-1 and the express of ICAM-1 in ascending aortic artery in psychological stress group were significantly increased than other groups(P<0.05 respectively).There was positive relation between the serum level of sICAM-1 and the express of ICAM-1 in ascending aortic samples(r=0.747,P<0.01).Conclusion(1) Psychological stress has an effect on both systemic and local inflammatory response.(2) It may be feasible for stress group to use serum inflammatory markers to deduce the inflammation in plaque,even to deduce its instability.(3) Treating psychological stress may prevent the development and the progression of vascular inflammation.