| 胃癌组织中EphB2受体胞外区结构域的克隆和序列测定 |
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| 引用本文： | 韩景田,陈小义,张晓光. 胃癌组织中EphB2受体胞外区结构域的克隆和序列测定[J]. 国际检验医学杂志, 2005, 26(3): 133-135 |
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| 作者姓名： | 韩景田 陈小义 张晓光 |
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| 作者单位： | 武警医学院中心实验室,天津,300162;武警医学院中心实验室,天津,300162;武警医学院中心实验室,天津,300162 |
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| 摘 要： | 目的对胃癌组织中酪氨酸蛋白激酶EphB2受体胞外区结构域进行克隆.方法从胃癌患者手术切片标本中提取RNA,进行RT-PCR,将扩增片段克隆入pUC19质粒,进行测序及酶切鉴定.结果克隆片段序列正确.结论为进行表达、筛选与之相互作用的蛋白,阐明其生理功能打下基础.
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| 关 键 词： | 酪氨酸蛋白激酶 EphB2 克隆 聚合酶链反应 |
| 修稿时间： | 2004-03-19 |
Cloning and sequencing of the ectodomain of EphB2 receptor in the tissue of gastric carcinoma |
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| HAN Jing-tian,CHEN Xiao-yi,ZHANG Xiao-guang. Cloning and sequencing of the ectodomain of EphB2 receptor in the tissue of gastric carcinoma[J]. International Journal of Laboratory Medicine, 2005, 26(3): 133-135 |
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| Authors: | HAN Jing-tian CHEN Xiao-yi ZHANG Xiao-guang |
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| Abstract: | Objective To clone the ectodomain of EphB 2 receptor tyrosine kinase in the tissue of gastric carcinoma.Methods Total RNA was extracted from a surgical specimen of human gastric carcinoma, and primers were designed by computer analysis and chemically synthesized. The amplified fragment was obtained by RT-PCR and cloned into pUC 19 vector. The recombinant clones were confirmed by sequencing and restriction endonuclease cleavages. Results The cloned fragment was identical to the design.Conclusion It will offer the basis for further investigation including identify its new ligand, the expression, and biological functions. |
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| Keywords: | Receptor tyrosine kinase EphB 2 Cloning Polymerase chain reaction(PCR) |
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