兔骨髓基质干细胞自体嚼肌内移植异位成骨实验研究

目的：观察骨髓基质干细胞成骨向分化诱导后植入嚼肌内异位成骨的过程。方法：从兔髂骨分离骨髓基质干细胞，经体外诱导分化，与磷酸三钙／羟基磷灰石（tricalcium phosphate／hydroxyapatite，TCP／HA）支架复合，植入兔自体嚼肌内，形态学、免疫细胞化学、组织学观察其成骨特征。结果：体外培养的兔骨髓基质干细胞性状稳定，经矿化诱导培养的骨髓基质干细胞表现为成骨细胞分化，表达Ⅰ型胶原蛋白，与支架材料复合植入自体嚼肌内可见成熟骨组织形成。结论：利用骨髓基质干细胞为种子细胞植入嚼肌内开展功能性组织工程化颌骨移植方法可行。

Experimental study on osteogenetic ability of rabbit bone marrow stem cells in autogenous masseter

objective:To evaluate osteogenesis of bone marrow stem cells (BMSCs) in combination with TCP/HA in vivo, providing a study model for bone tissue engineering.Method:BMSCs were obtained from New Zealand White rabbit. Biological characteristics of BMSCs were identified by morphology and immunocytochemistry. Meanwhile, the explants of BMSCs and TCP/HA ,implanted into autogenous masseter of the rabbit, were observed by histology 14 weeks later.Result:BMSCs were cultured successfully. The result of immunocytochemical stain showed that BMSCs expressed collagen I. Mineralization nodules were demonstrated after 14 days of induction with mineralization medium. The explants of BMSCs and TCP/HA could product bone tissue.Conclusion:Transplant of BMSCs in combination with TCP/HA is a favorable bone tissue engineering way.